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Correlation between compartmental tenofovir concentrations and an ex vivo rectal biopsy model of tissue infectibility in the RMP-02/MTN-006 phase 1 study

Richardson-Harman, N and Hendrix, CW and Bumpus, NN and Mauck, C and Cranston, RD and Yang, K and Elliott, J and Tanner, K and McGowan, I and Kashuba, A and Anton, PA (2014) Correlation between compartmental tenofovir concentrations and an ex vivo rectal biopsy model of tissue infectibility in the RMP-02/MTN-006 phase 1 study. PLoS ONE, 9 (10).

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Abstract

© 2014 Richardson-Harman et al. Design: Phase 1, randomized, two-site (US), double-blind, placebo-controlled study of sexually-abstinent males and females.Methods: Eighteen participants received a single 300 mg exposure of oral TDF and were then randomized 2:1 to receive a single then seven-daily rectal exposures of TFV 1% gel (40 mg TFV per 4 ml gel application) or hydroxyethyl-cellulose (HEC) placebo gel. Blood and rectal biopsies were collected for pharmacokinetic TDF and TFVdp analyses and ex vivo HIV-1 challenge.Results: There was a significant fit for the TFVdp dose-response model for rectal tissue (p = 0.0004), CD4+MMC (p<0.0001), CD42 MMC (p<0.0001), and TotalMMC (p<0.0001) compartments with r2 ranging 0.36-0.64. Higher concentrations of TFVdp corresponded with lower p24, consistent with drug-mediated virus suppression. The single oral treatment failed to provide adequate compartment drug exposure to reach the EC50 of rectal tissue TFVdp predicted to be necessary to suppress HIV in rectal tissue. The EC50 for CD4+MMC was within the single topical treatment range, providing evidence that a 1% topical, vaginally-formulated TFV gel provided in-vivo doses predicted to provide for 50% efficacy in the ex vivo assay. The 7-daily topical TFV gel treatment provided TFVdp concentrations that reached EC90 biopsy efficacy for CD4-MMC, CD4+MMC and TotalMMC compartments.Conclusion: The TFVdp MMC compartment (CD4+, CD4- and Total) provided the best surrogate for biopsy infectibility and the 7-daily topical TFV gel treatment provided the strongest PK profile for HIV suppression.Objectives: This study was designed to assess the dose-response relationship between tissue, blood, vaginal and rectal compartment concentrations of tenofovir (TFV) and tenofovir diphosphate (TFVdp) and ex vivo rectal HIV suppression following oral tenofovir disoproxil fumarate (TDF) and rectal administration of TFV 1% vaginally-formulated gel. Copyright:


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Details

Item Type: Article
Status: Published
Creators/Authors:
CreatorsEmailPitt UsernameORCID
Richardson-Harman, N
Hendrix, CW
Bumpus, NN
Mauck, C
Cranston, RDrdc27@pitt.eduRDC27
Yang, K
Elliott, J
Tanner, K
McGowan, Iimcgowan@pitt.eduIMCGOWAN
Kashuba, A
Anton, PA
Contributors:
ContributionContributors NameEmailPitt UsernameORCID
EditorWinston, AlanUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Centers: Other Centers, Institutes, Offices, or Units > Magee-Women's Research Institute
Date: 28 October 2014
Date Type: Publication
Journal or Publication Title: PLoS ONE
Volume: 9
Number: 10
DOI or Unique Handle: 10.1371/journal.pone.0111507
Schools and Programs: School of Medicine > Medicine
Refereed: Yes
Other ID: NLM PMC4211741
PubMed Central ID: PMC4211741
PubMed ID: 25350130
Date Deposited: 12 May 2015 18:26
Last Modified: 27 Oct 2018 14:55
URI: http://d-scholarship.pitt.edu/id/eprint/24037

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