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Compartment-Specific and Sequential Role of MyD88 and CARD9 in Chemokine Induction and Innate Defense during Respiratory Fungal Infection

Jhingran, A and Kasahara, S and Shepardson, KM and Junecko, BAF and Heung, LJ and Kumasaka, DK and Knoblaugh, SE and Lin, X and Kazmierczak, BI and Reinhart, TA and Cramer, RA and Hohl, TM (2015) Compartment-Specific and Sequential Role of MyD88 and CARD9 in Chemokine Induction and Innate Defense during Respiratory Fungal Infection. PLoS Pathogens, 11 (1). 1 - 22. ISSN 1553-7366

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Abstract

Aspergillus fumigatus forms ubiquitous airborne conidia that humans inhale on a daily basis. Although respiratory fungal infection activates the adaptor proteins CARD9 and MyD88 via C-type lectin, Toll-like, and interleukin-1 family receptor signals, defining the temporal and spatial pattern of MyD88- and CARD9-coupled signals in immune activation and fungal clearance has been difficult to achieve. Herein, we demonstrate that MyD88 and CARD9 act in two discrete phases and in two cellular compartments to direct chemokine- and neutrophil-dependent host defense. The first phase depends on MyD88 signaling because genetic deletion of MyD88 leads to delayed induction of the neutrophil chemokines CXCL1 and CXCL5, delayed neutrophil lung trafficking, and fatal pulmonary damage at the onset of respiratory fungal infection. MyD88 expression in lung epithelial cells restores rapid chemokine induction and neutrophil recruitment via interleukin-1 receptor signaling. Exogenous CXCL1 administration reverses murine mortality in MyD88-deficient mice. The second phase depends predominately on CARD9 signaling because genetic deletion of CARD9 in radiosensitive hematopoietic cells interrupts CXCL1 and CXCL2 production and lung neutrophil recruitment beyond the initial MyD88-dependent phase. Using a CXCL2 reporter mouse, we show that lung-infiltrating neutrophils represent the major cellular source of CXCL2 during CARD9-dependent recruitment. Although neutrophil-intrinsic MyD88 and CARD9 function are dispensable for neutrophil conidial uptake and killing in the lung, global deletion of both adaptor proteins triggers rapidly progressive invasive disease when mice are challenged with an inoculum that is sub-lethal for single adapter protein knockout mice. Our findings demonstrate that distinct signal transduction pathways in the respiratory epithelium and hematopoietic compartment partially overlap to ensure optimal chemokine induction, neutrophil recruitment, and fungal clearance within the respiratory tract.

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Details

Item Type: Article
Status: Published
Creators/Authors:
CreatorsEmailPitt UsernameORCID
Jhingran, A
Kasahara, S
Shepardson, KM
Junecko, BAF
Heung, LJ
Kumasaka, DK
Knoblaugh, SE
Lin, X
Kazmierczak, BI
Reinhart, TAreinhar@pitt.eduREINHAR
Cramer, RA
Hohl, TM
Contributors:
ContributionContributors NameEmailPitt UsernameORCID
EditorKlein, Bruce S.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Date: 1 January 2015
Date Type: Publication
Journal or Publication Title: PLoS Pathogens
Volume: 11
Number: 1
Page Range: 1 - 22
DOI or Unique Handle: 10.1371/journal.ppat.1004589
Schools and Programs: School of Public Health > Infectious Diseases and Microbiology
Refereed: Yes
ISSN: 1553-7366
Other ID: NLM PMC4306481
PubMed Central ID: PMC4306481
PubMed ID: 25621893
Date Deposited: 12 May 2015 19:50
Last Modified: 30 Mar 2021 11:55
URI: http://d-scholarship.pitt.edu/id/eprint/24082

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