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Beryllium increases the CD14<sup>dim</sup>CD16+ subset in the lung of chronic beryllium disease

Li, L and Hamzeh, N and Gillespie, M and Elliott, J and Wang, J and Gottschall, EB and Mroz, PM and Maier, LA (2015) Beryllium increases the CD14<sup>dim</sup>CD16+ subset in the lung of chronic beryllium disease. PLoS ONE, 10 (2).

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Abstract

© 2015 Li et al. CD14<sup>dim</sup>CD16+ and CD14<sup>bright</sup>CD16+ cells, which compose a minor population of monocytes in human peripheral blood mononuclear cells (PBMC), have been implicated in several inflammatory diseases. The aim of this study was to investigate whether this phenotype was present as a subset of lung infiltrative alveolar macrophages (AMs) in the granulomatous lung disease, chronic beryllium disease (CBD). The monocytes subsets was determined from PBMC cells and bronchoalveolar lavage (BAL) cells from CBD, beryllium sensitized Non-smoker (BeS-NS) and healthy subjects (HS) using flow cytometry. The impact of smoking on the AMs cell phenotype was determined by using BAL cells from BeS smokers (BeS-S). In comparison with the other monocyte subpopulations, CD14<sup>dim</sup>CD16+ cells were at decreased frequency in PBMCs of both BeS-NS and CBD and showed higher HLA-DR expression, compared to HS. The AMs from CBD and BeS-NS demonstrated a CD14<sup>dim</sup>CD16+phenotype, while CD14<sup>bright</sup>CD16+ cells were found at increased frequency in AMs of BeS, compared to HS. Fresh AMs from BeS-NS and CBD demonstrated significantly greater CD16, CD40, CD86 and HLA-DR than HS and BeS-S. The expression of CD16 on AMs from both CBD and BeS-NS was downregulated significantly after 10μM BeSO<inf>4</inf> stimulation. The phagocytic activity of AMs decreased after 10μM BeSO<inf>4</inf> treatment in both BeS-NS and CBD, although was altered or reduced in HS and BeS-S. These results suggest that Be increases the CD14<sup>dim</sup>CD16+ subsets in the lung of CBD subjects. We speculate that Be-stimulates the compartmentalization of a more mature CD16+ macrophage phenotype and that in turn these macrophages are a source of Th1 cytokines and chemokines that perpetuate the Be immune response in CBD. The protective effect of cigarette smoking in BeS-S may be due to the low expression of co-stimulatory markers on AMs from smokers as well as the decreased phagocytic function.


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Details

Item Type: Article
Status: Published
Creators/Authors:
CreatorsEmailPitt UsernameORCID
Li, L
Hamzeh, N
Gillespie, M
Elliott, J
Wang, Jjiw95@pitt.eduJIW95
Gottschall, EB
Mroz, PM
Maier, LA
Contributors:
ContributionContributors NameEmailPitt UsernameORCID
EditorCrouser, Elliott D.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Date: 17 February 2015
Date Type: Publication
Journal or Publication Title: PLoS ONE
Volume: 10
Number: 2
DOI or Unique Handle: 10.1371/journal.pone.0117276
Schools and Programs: School of Medicine > Pediatrics
Refereed: Yes
Other ID: NLM PMC4331542
PubMed Central ID: PMC4331542
PubMed ID: 25689051
Date Deposited: 12 May 2015 18:12
Last Modified: 02 Feb 2019 16:58
URI: http://d-scholarship.pitt.edu/id/eprint/24096

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