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Distinct human stem cell populations in small and large intestine

Cramer, JM and Thompson, T and Geskin, A and Laframboise, W and Lagasse, E (2015) Distinct human stem cell populations in small and large intestine. PLoS ONE, 10 (3).

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Abstract

© 2015 Cramer et al. The intestine is composed of an epithelial layer containing rapidly proliferating cells that mature into two regions, the small and the large intestine. Although previous studies have identified stem cells as the cell-of-origin for intestinal epithelial cells, no studies have directly compared stem cells derived from these anatomically distinct regions. Here, we examine intrinsic differences between primary epithelial cells isolated from human fetal small and large intestine, after in vitro expansion, using the Wnt agonist R-spondin 2.We utilized flow cytometry, fluorescence-activated cell sorting, gene expression analysis and a three-dimensional in vitro differentiation assay to characterize their stemcell properties. We identified stem cell markers that separate subpopulations of colony-forming cells in the small and large intestine and revealed important differences in differentiation, proliferation and disease pathways using gene expression analysis. Single cells from small and large intestine cultures formed organoids that reflect the distinct cellular hierarchy found in vivo and respond differently to identical exogenous cues. Our characterization identified numerous differences between small and large intestine epithelial stem cells suggesting possible connections to intestinal disease.


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Details

Item Type: Article
Status: Published
Creators/Authors:
CreatorsEmailPitt UsernameORCID
Cramer, JM
Thompson, T
Geskin, A
Laframboise, Wwal9@pitt.eduWAL90000-0002-6024-810X
Lagasse, Elagasse@pitt.eduLAGASSE
Contributors:
ContributionContributors NameEmailPitt UsernameORCID
EditorSun, JunUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Centers: Other Centers, Institutes, or Units > McGowan Institute for Regenerative Medicine
Date: 9 March 2015
Date Type: Publication
Journal or Publication Title: PLoS ONE
Volume: 10
Number: 3
DOI or Unique Handle: 10.1371/journal.pone.0118792
Schools and Programs: School of Medicine > Pathology
Swanson School of Engineering > Bioengineering
Refereed: Yes
PubMed ID: 25751518
Date Deposited: 12 May 2015 18:08
Last Modified: 04 Feb 2019 19:55
URI: http://d-scholarship.pitt.edu/id/eprint/24102

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