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Evolution and probable transmission of intersubtype recombinant human immunodeficiency virus type 1 in a Zambian couple

Salminen, MO and Carr, JK and Robertson, DL and Hegerich, P and Gotte, D and Koch, C and Sanders-Buell, E and Gao, F and Sharp, PM and Hahn, BH and Burke, DS and McCutchan, FE (1997) Evolution and probable transmission of intersubtype recombinant human immunodeficiency virus type 1 in a Zambian couple. Journal of Virology, 71 (4). 2647 - 2655. ISSN 0022-538X

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The extraordinary genetic diversity of human immunodeficiency virus type 1 (HIV-1) results from the introduction of mutations by an error-prone reverse transcriptase and from recombination of the two RNA genomes packaged in the virion during the synthesis of proviral DNA. The occurrence of multiple, genetically distant HIV-1 subtypes and their geographic intermixing set up conditions for dramatic, rather than gradual, changes in genotype whenever genomes from different subtypes are copackaged in virions. Here we describe, for the first time, the sequential generation of multiple different, but related, intersubtype HIV-1 recombinants within an infected individual. Full-length gag and env genes were recovered directly from peripheral blood mononuclear cells or from primary virus cultures, using serial blood samples from a Zambian woman and a sample from her spouse. DNA sequencing and phylogenetic analysis established that two different A/C recombinant forms of HIV-1 predominated at two time points in the woman. A related but distinct recombinant HIV-I was recovered from her spouse. Intersubtype recombination apparently played a central role in the evolution of HIV-1 in this couple and may contribute substantially to the rapid emergence of HIV-1 variants whenever mixed-subtype HIV-1 infections occur.


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Item Type: Article
Status: Published
CreatorsEmailPitt UsernameORCID
Salminen, MO
Carr, JK
Robertson, DL
Hegerich, P
Gotte, D
Koch, C
Sanders-Buell, E
Gao, F
Sharp, PM
Hahn, BH
Burke, DSdonburke@pitt.eduDONBURKE
McCutchan, FE
Centers: Other Centers, Institutes, Offices, or Units > Center for Vaccine Research
Date: 1 January 1997
Date Type: Publication
Access Restriction: No restriction; Release the ETD for access worldwide immediately.
Journal or Publication Title: Journal of Virology
Volume: 71
Number: 4
Page Range: 2647 - 2655
DOI or Unique Handle: 10.1128/jvi.71.4.2647-2655.1997
Institution: University of Pittsburgh
Schools and Programs: School of Public Health > Epidemiology
Refereed: Yes
ISSN: 0022-538X
Date Deposited: 08 May 2015 15:13
Last Modified: 02 Aug 2020 14:56


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