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Full-length sequence of an Ethiopian human immunodeficiency virus type 1 (HIV-1) isolate of genetic subtype C

Salminen, MO and Johansson, B and Sönnerborg, A and Ayehunie, S and Gotte, D and Leinikki, P and Burke, DS and McCutchan, FE (1996) Full-length sequence of an Ethiopian human immunodeficiency virus type 1 (HIV-1) isolate of genetic subtype C. AIDS Research and Human Retroviruses, 12 (14). 1329 - 1339. ISSN 0889-2229

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Genetic subtype C of the human immunodeficiency virus type-1 (HIV-1) has established foci of infection in India and in at least eight African countries, and is expected to contribute significantly to the global pandemic. Here we report the first almost full-length sequence of a subtype C HIV-1 from Ethiopia. Clone C2220, 9031 nt in length, was derived by long PCR amplification of proviral DNA from virus cultured on primary peripheral blood mononuclear cells, and contains all but 74 nt of the unique sequence information of the HIV-1 genome. This clone resembles HIV-1 isolates of subtypes A, B, and D in its genome organization with one notable exception: the core promoter contains not two, but three potential binding sites for the transcription factor NF-kB. The extra NF-kB site was found in all other Ethiopian strains analyzed, as well as in subtype C viruses from Zambia, suggesting it is typical for the C-subtype of HIV-1. The phylogenetic relationship of C2220 to other HIV-1 isolates is also presented. Subtype C viruses circulating in Ethiopia exhibit the low interisolate diversity typical of other, newly established HIV-1 epidemics, and C2220 is both representative of Ethiopian subtype C viruses and a suitable prototype for the development of vaccines against HIV-1 subtype C.


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Item Type: Article
Status: Published
CreatorsEmailPitt UsernameORCID
Salminen, MO
Johansson, B
Sönnerborg, A
Ayehunie, S
Gotte, D
Leinikki, P
Burke, DSdonburke@pitt.eduDONBURKE
McCutchan, FE
Centers: Other Centers, Institutes, Offices, or Units > Center for Vaccine Research
Date: 20 September 1996
Date Type: Publication
Access Restriction: No restriction; Release the ETD for access worldwide immediately.
Journal or Publication Title: AIDS Research and Human Retroviruses
Volume: 12
Number: 14
Page Range: 1329 - 1339
DOI or Unique Handle: 10.1089/aid.1996.12.1329
Institution: University of Pittsburgh
Schools and Programs: School of Public Health > Epidemiology
Refereed: Yes
ISSN: 0889-2229
Date Deposited: 08 May 2015 15:22
Last Modified: 02 Feb 2019 16:57


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