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Human immunodeficiency virus type 1 neutralizing antibody serotyping using serum pools and an infectivity reduction assay

Mascola, JR and Louder, MK and Surman, SR and Vancott, TC and Yu, XF and Bradac, J and Porter, KR and Nelson, KE and Girard, M and McNeil, JG and McCutchan, FE and Birx, DL and Burke, DS (1996) Human immunodeficiency virus type 1 neutralizing antibody serotyping using serum pools and an infectivity reduction assay. AIDS Research and Human Retroviruses, 12 (14). 1319 - 1328. ISSN 0889-2229

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Abstract

Classification of human immunodeficiency virus type 1 (HIV-1) by neutralization serotype may be important for the design of active and passive immunization strategies. Neutralizing antibody serotyping is hindered by the lack of standard reagents and assay format, and by the weak activity of many individual sera. To facilitate cross-clade neutralization analysis, we used an infectivity reduction assay (IRA) and selected clade-specific serum (or plasma) pools from subjects infected with clade B and E HIV-1, respectively. Several serum pools were utilized; some were selected for strong neutralizing activity against intraclade viruses and others were derived from conveniently available samples. Against a panel of 51 clade B and E viruses, serum pools displayed strong neutralization of most intraclade viruses and significantly diminished cross-clade neutralization. Results were confirmed against a blinded panel of 20 viruses. The data indicate that the phylogenetic classification of virus subtypes B and E corresponds to two distinct neutralization serotypes. This approach to neutralizing antibody serotyping may be useful in defining the antigenic relationship among viruses from other clades.


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Details

Item Type: Article
Status: Published
Creators/Authors:
CreatorsEmailPitt UsernameORCID
Mascola, JR
Louder, MK
Surman, SR
Vancott, TC
Yu, XF
Bradac, J
Porter, KR
Nelson, KE
Girard, M
McNeil, JG
McCutchan, FE
Birx, DL
Burke, DSdonburke@pitt.eduDONBURKE
Centers: Other Centers, Institutes, or Units > Center for Vaccine Research
Date: 20 September 1996
Date Type: Publication
Access Restriction: No restriction; Release the ETD for access worldwide immediately.
Journal or Publication Title: AIDS Research and Human Retroviruses
Volume: 12
Number: 14
Page Range: 1319 - 1328
DOI or Unique Handle: 10.1089/aid.1996.12.1319
Institution: University of Pittsburgh
Schools and Programs: Graduate School of Public Health > Epidemiology
Refereed: Yes
ISSN: 0889-2229
Date Deposited: 08 May 2015 15:11
Last Modified: 02 Feb 2019 16:57
URI: http://d-scholarship.pitt.edu/id/eprint/24337

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