Mascola, JR and Louder, MK and Surman, SR and Vancott, TC and Yu, XF and Bradac, J and Porter, KR and Nelson, KE and Girard, M and McNeil, JG and McCutchan, FE and Birx, DL and Burke, DS
(1996)
Human immunodeficiency virus type 1 neutralizing antibody serotyping using serum pools and an infectivity reduction assay.
AIDS Research and Human Retroviruses, 12 (14).
1319 - 1328.
ISSN 0889-2229
![[img]](http://d-scholarship.pitt.edu/style/images/fileicons/text_plain.png) |
Plain Text (licence)
Available under License : See the attached license file.
Download (1kB)
|
Abstract
Classification of human immunodeficiency virus type 1 (HIV-1) by neutralization serotype may be important for the design of active and passive immunization strategies. Neutralizing antibody serotyping is hindered by the lack of standard reagents and assay format, and by the weak activity of many individual sera. To facilitate cross-clade neutralization analysis, we used an infectivity reduction assay (IRA) and selected clade-specific serum (or plasma) pools from subjects infected with clade B and E HIV-1, respectively. Several serum pools were utilized; some were selected for strong neutralizing activity against intraclade viruses and others were derived from conveniently available samples. Against a panel of 51 clade B and E viruses, serum pools displayed strong neutralization of most intraclade viruses and significantly diminished cross-clade neutralization. Results were confirmed against a blinded panel of 20 viruses. The data indicate that the phylogenetic classification of virus subtypes B and E corresponds to two distinct neutralization serotypes. This approach to neutralizing antibody serotyping may be useful in defining the antigenic relationship among viruses from other clades.
Share
Citation/Export: |
|
Social Networking: |
|
Details
Item Type: |
Article
|
Status: |
Published |
Creators/Authors: |
Creators | Email | Pitt Username | ORCID  |
---|
Mascola, JR | | | | Louder, MK | | | | Surman, SR | | | | Vancott, TC | | | | Yu, XF | | | | Bradac, J | | | | Porter, KR | | | | Nelson, KE | | | | Girard, M | | | | McNeil, JG | | | | McCutchan, FE | | | | Birx, DL | | | | Burke, DS | donburke@pitt.edu | DONBURKE | |
|
Centers: |
Other Centers, Institutes, Offices, or Units > Center for Vaccine Research |
Date: |
20 September 1996 |
Date Type: |
Publication |
Access Restriction: |
No restriction; Release the ETD for access worldwide immediately. |
Journal or Publication Title: |
AIDS Research and Human Retroviruses |
Volume: |
12 |
Number: |
14 |
Page Range: |
1319 - 1328 |
DOI or Unique Handle: |
10.1089/aid.1996.12.1319 |
Institution: |
University of Pittsburgh |
Schools and Programs: |
School of Public Health > Epidemiology |
Refereed: |
Yes |
ISSN: |
0889-2229 |
Date Deposited: |
08 May 2015 15:11 |
Last Modified: |
02 Feb 2019 16:57 |
URI: |
http://d-scholarship.pitt.edu/id/eprint/24337 |
Metrics
Monthly Views for the past 3 years
Plum Analytics
Altmetric.com
Actions (login required)
 |
View Item |