Sabbaj, S and Mulligan, MJ and Hsieh, RH and Belshe, RB and McGhee, JR and Schwartz, D and Burke, D and Gorse, GJ and Frey, S and Mestecky, J and Jackson, S and Gnann, JW and Goepfert, PA and Dolin, R and Keefer, MC and Evans, TG and McElrath, MJ and Corey, L and Graham, BS and Wright, P and Spearman, P and Matthews, T and Montefiori, D and Weinhold, K and Bolognesi, D and Allen, M and Savarese, B
(2000)
Cytokine profiles in seronegative volunteers immunized with a recombinant canarypox and gp120 prime-boost HIV-1 vaccine.
AIDS, 14 (10).
1365 - 1374.
ISSN 0269-9370
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Abstract
Objectives: To study memory T cell proliferative responses and cytokine profiles induced in HIV-1 seronegative volunteers immunized with a live recombinant canarypox vector expressing HIV-1 antigens (ALVAC-HIV) and boosted with a recombinant gp120 subunit vaccine. Design: HIV-specific T cell proliferative responses and cytokines were measured 2 weeks after vaccination. Cytokines secreted by T helper 1 cells (Th1) [interleukin (IL)-2 and interferon-γ (IFN-γ)] and T helper 2 (Th2) cells (IL-4, IL-5, IL-6, and IL-10) were assessed both at the mRNA and the protein level. Methods: Peripheral blood mononuclear cells (PBMC) were stimulated in vitro with HIV antigens. Subsequently, T cell proliferation was measured in a standard lymphoproliferation assay; secreted cytokines were measured using an enzyme-linked immunosorbent assay and upregulation of cytokine mRNA was measured using reverse transcriptase polymerase chain reaction. Results: All individuals who had received ALVAC-HIV followed by the protein vaccine exhibited HIV-1-specific T cell proliferative responses. Moreover, the PBMC of all prime-boost vaccinated individuals produced detectable IFN-γ and IL-10 in response to stimulation with HIV-1 envelope glycoprotein antigens; 83% also had detectable levels of IL-2 and IL-6, 71% had detectable levels of IL-4, and 86% had detectable levels of IL-5. Conclusions: These data indicate that this vaccination regimen was inducing both Th1- and Th2-type responses to HIV-1 envelope antigens. This prime-boost vaccination approach elicited T cell help for the generation of cytotoxic T lymphocyte responses as well as help for antibody production and so promises to generate a broad HIV-1-specific immune response. (C) 2000 Lippincott Williams and Wilkins.
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Item Type: |
Article
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Status: |
Published |
Creators/Authors: |
Creators | Email | Pitt Username | ORCID  |
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Sabbaj, S | | | | Mulligan, MJ | | | | Hsieh, RH | | | | Belshe, RB | | | | McGhee, JR | | | | Schwartz, D | | | | Burke, D | donburke@pitt.edu | DONBURKE | | Gorse, GJ | | | | Frey, S | | | | Mestecky, J | | | | Jackson, S | | | | Gnann, JW | | | | Goepfert, PA | | | | Dolin, R | | | | Keefer, MC | | | | Evans, TG | | | | McElrath, MJ | | | | Corey, L | | | | Graham, BS | | | | Wright, P | | | | Spearman, P | | | | Matthews, T | | | | Montefiori, D | | | | Weinhold, K | | | | Bolognesi, D | | | | Allen, M | | | | Savarese, B | | | |
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Centers: |
Other Centers, Institutes, Offices, or Units > Center for Vaccine Research |
Date: |
28 July 2000 |
Date Type: |
Publication |
Journal or Publication Title: |
AIDS |
Volume: |
14 |
Number: |
10 |
Page Range: |
1365 - 1374 |
DOI or Unique Handle: |
10.1097/00002030-200007070-00009 |
Schools and Programs: |
School of Public Health > Epidemiology |
Refereed: |
Yes |
ISSN: |
0269-9370 |
Date Deposited: |
07 May 2015 19:38 |
Last Modified: |
02 Feb 2019 16:57 |
URI: |
http://d-scholarship.pitt.edu/id/eprint/24397 |
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