Cytokine profiles in seronegative volunteers immunized with a recombinant canarypox and gp120 prime-boost HIV-1 vaccine

Sabbaj, S and Mulligan, MJ and Hsieh, RH and Belshe, RB and McGhee, JR and Schwartz, D and Burke, D and Gorse, GJ and Frey, S and Mestecky, J and Jackson, S and Gnann, JW and Goepfert, PA and Dolin, R and Keefer, MC and Evans, TG and McElrath, MJ and Corey, L and Graham, BS and Wright, P and Spearman, P and Matthews, T and Montefiori, D and Weinhold, K and Bolognesi, D and Allen, M and Savarese, B (2000) Cytokine profiles in seronegative volunteers immunized with a recombinant canarypox and gp120 prime-boost HIV-1 vaccine. AIDS, 14 (10). 1365 - 1374. ISSN 0269-9370

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Abstract

Objectives: To study memory T cell proliferative responses and cytokine profiles induced in HIV-1 seronegative volunteers immunized with a live recombinant canarypox vector expressing HIV-1 antigens (ALVAC-HIV) and boosted with a recombinant gp120 subunit vaccine. Design: HIV-specific T cell proliferative responses and cytokines were measured 2 weeks after vaccination. Cytokines secreted by T helper 1 cells (Th1) [interleukin (IL)-2 and interferon-γ (IFN-γ)] and T helper 2 (Th2) cells (IL-4, IL-5, IL-6, and IL-10) were assessed both at the mRNA and the protein level. Methods: Peripheral blood mononuclear cells (PBMC) were stimulated in vitro with HIV antigens. Subsequently, T cell proliferation was measured in a standard lymphoproliferation assay; secreted cytokines were measured using an enzyme-linked immunosorbent assay and upregulation of cytokine mRNA was measured using reverse transcriptase polymerase chain reaction. Results: All individuals who had received ALVAC-HIV followed by the protein vaccine exhibited HIV-1-specific T cell proliferative responses. Moreover, the PBMC of all prime-boost vaccinated individuals produced detectable IFN-γ and IL-10 in response to stimulation with HIV-1 envelope glycoprotein antigens; 83% also had detectable levels of IL-2 and IL-6, 71% had detectable levels of IL-4, and 86% had detectable levels of IL-5. Conclusions: These data indicate that this vaccination regimen was inducing both Th1- and Th2-type responses to HIV-1 envelope antigens. This prime-boost vaccination approach elicited T cell help for the generation of cytotoxic T lymphocyte responses as well as help for antibody production and so promises to generate a broad HIV-1-specific immune response. (C) 2000 Lippincott Williams and Wilkins.

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Details

Item Type:	Article
Status:	Published
Creators/Authors:	Creators Email Pitt Username ORCID Sabbaj, S Mulligan, MJ Hsieh, RH Belshe, RB McGhee, JR Schwartz, D Burke, D donburke@pitt.edu DONBURKE Gorse, GJ Frey, S Mestecky, J Jackson, S Gnann, JW Goepfert, PA Dolin, R Keefer, MC Evans, TG McElrath, MJ Corey, L Graham, BS Wright, P Spearman, P Matthews, T Montefiori, D Weinhold, K Bolognesi, D Allen, M Savarese, B
Centers:	Other Centers, Institutes, Offices, or Units > Center for Vaccine Research
Date:	28 July 2000
Date Type:	Publication
Journal or Publication Title:	AIDS
Volume:	14
Number:	10
Page Range:	1365 - 1374
DOI or Unique Handle:	10.1097/00002030-200007070-00009
Schools and Programs:	School of Public Health > Epidemiology
Refereed:	Yes
ISSN:	0269-9370
Date Deposited:	07 May 2015 19:38
Last Modified:	02 Feb 2019 16:57
URI:	http://d-scholarship.pitt.edu/id/eprint/24397

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