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V3 seroreactivity and sequence variation: Tracking the emergence of V3 genotypic variation in HIV-1-infected patients

Michael, NL and Davis, KE and Loomis-Price, LD and VanCott, TC and Burke, DS and Redfield, RR and Birx, DL (1996) V3 seroreactivity and sequence variation: Tracking the emergence of V3 genotypic variation in HIV-1-infected patients. AIDS, 10 (2). 121 - 129. ISSN 0269-9370

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Abstract

Objective: To investigate the relationship between V3-specific immune responses and viral quasispecies evolution in 10 HIV-1-seropositive patients enrolled in a phase I trial of recombinant gp160. Methods: Serologic responses to the HIV(LAI) V3 loop and autologous V3 loop DNA sequences were sequentially determined over a 3-4-year interval. Results: Six patients either seroconverted or had a ≥ 42-fold boost in titer to the V3 reagent associated with an average of 3.2 amino-acid changes in their autologous V3 loops. Four patients with ≤ 11-fold change in titer to the V3 loop showed an average of 0.75 amino-acid changes. Attempts to measure autologous V3 loop responses in four patients using a peptide enzyme-linked immunosorbent assay technique did not show a distinct binding preference for autologous versus heterologous V3 loop peptides. Thus, we interpret seroreactivity to the heterologous HIV(LAI) V3 loop to reflect the broadness of the V3 immune response rather than a direct measure of epitope-specific Immune pressure. Conclusions: These data suggest that the broadness of serologic responses to viral epitopes are reflected in the rate of evolution of their cognate coding sequences and support the view that the immune response to HIV-1 results in the continuous selection of new viral variants during the course of disease.


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Details

Item Type: Article
Status: Published
Creators/Authors:
CreatorsEmailPitt UsernameORCID
Michael, NL
Davis, KE
Loomis-Price, LD
VanCott, TC
Burke, DSdonburke@pitt.eduDONBURKE
Redfield, RR
Birx, DL
Centers: Other Centers, Institutes, or Units > Center for Vaccine Research
Date: 1 January 1996
Date Type: Publication
Access Restriction: No restriction; Release the ETD for access worldwide immediately.
Journal or Publication Title: AIDS
Volume: 10
Number: 2
Page Range: 121 - 129
DOI or Unique Handle: 10.1097/00002030-199602000-00001
Institution: University of Pittsburgh
Schools and Programs: Graduate School of Public Health > Epidemiology
Refereed: Yes
ISSN: 0269-9370
Date Deposited: 07 May 2015 19:23
Last Modified: 02 Feb 2019 16:57
URI: http://d-scholarship.pitt.edu/id/eprint/24430

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