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HLA class I diversity among rural rainforest inhabitants in Cameroon: Identification of A*2612-B*4407 haplotype

Torimiro, JN and Carr, JK and Wolfe, ND and Karacki, P and Martin, MP and Gao, X and Tamoufe, U and Thomas, A and Ngole, EM and Birx, DL and McCutchan, FE and Burke, DS and Carrington, M (2006) HLA class I diversity among rural rainforest inhabitants in Cameroon: Identification of A*2612-B*4407 haplotype. Tissue Antigens, 67 (1). 30 - 37. ISSN 0001-2815

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Abstract

The population distribution of alleles of the classical HLA class I loci in Cameroon has not been well studied but is of particular interest given the AIDS and malarial epidemics afflicting this population. We investigated the genetic diversity of HLA-A, HLA-B and HLA-C alleles in remote populations of Cameroon. Subjects from seven small, isolated, indigenous populations (N = 274) in the rainforest of southern Cameroon were typed for HLA-A, HLA-B and HLA-C alleles using a polymerase chain reaction/sequence-specific oligonucleotide probe assay and sequence analysis. Multiple alleles of the HLA-A (N = 28), HLA-B (N = 41) and HLA-C (N = 21) loci were identified, of which A*2301 [allele frequency (AF) = 12.8%], B*5802 (AF = 10.9%) and Cw*0401 (AF = 16.6%) were the most frequent individual alleles and A*02 (AF = 19.0%), B*58 (AF = 15.9%) and Cw*07 (AF = 22.4%) the most common serologically defined groups of alleles. Twenty-six (28.9%) alleles with a frequency of less than 1% (AF < 1%), 39 (43%) with a frequency of 2.0-15.0% (AF = 2.0-15.0%), three globally uncommon alleles [A*2612 (AF = 2.0%), B*4016 (AF = 0.7%) and B*4407 (AF = 1.4%)], and the A*2612-Cw*0701/06/18- B*4407 haplotype (haplotype frequency = 1.3%) were also identified. Heterozygosity values of 0.89, 0.92 and 0.89 were determined for HLA-A, HLA-B and HLA-C, respectively. The extensive allelic and haplotypic diversity observed in this population may have resulted from varied natural selective pressures on the population, as well as intermingling of peoples from multiple origins. Thus, from an anthropologic perspective, these data highlight the challenges in T-cell-based vaccine development, the identification of allogeneic transplant donors and the understanding of infectious disease patterns in different populations. © 2006 Blackwell Munksgaard.


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Details

Item Type: Article
Status: Published
Creators/Authors:
CreatorsEmailPitt UsernameORCID
Torimiro, JN
Carr, JK
Wolfe, ND
Karacki, P
Martin, MP
Gao, X
Tamoufe, U
Thomas, A
Ngole, EM
Birx, DL
McCutchan, FE
Burke, DSdonburke@pitt.eduDONBURKE
Carrington, M
Centers: Other Centers, Institutes, Offices, or Units > Center for Vaccine Research
Date: 1 January 2006
Date Type: Publication
Journal or Publication Title: Tissue Antigens
Volume: 67
Number: 1
Page Range: 30 - 37
DOI or Unique Handle: 10.1111/j.1399-0039.2005.00527.x
Schools and Programs: Graduate School of Public Health > Epidemiology
Refereed: Yes
ISSN: 0001-2815
Date Deposited: 07 May 2015 19:22
Last Modified: 02 Feb 2019 16:57
URI: http://d-scholarship.pitt.edu/id/eprint/24440

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