Oh, SK and Stegman, B and Pendleton, CD and Ota, MO and Pan, CH and Griffin, DE and Burke, DS and Berzofsky, JA
(2006)
Protective immunity provided by HLA-A2 epitopes for fusion and hemagglutinin proteins of measles virus.
Virology, 352 (2).
390 - 399.
ISSN 0042-6822
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Abstract
Natural infection and vaccination with a live-attenuated measles virus (MV) induce CD8+ T-cell-mediated immune responses that may play a central role in controlling MV infection. In this study, we show that newly identified human HLA-A2 epitopes from MV hemagglutinin (H) and fusion (F) proteins induced protective immunity in HLA-A2 transgenic mice challenged with recombinant vaccinia viruses expressing F or H protein. HLA-A2 epitopes were predicted and synthesized. Five and four peptides from H and F, respectively, bound to HLA-A2 molecules in a T2-binding assay, and four from H and two from F could induce peptide-specific CD8+ T cell responses in HLA-A2 transgenic mice. Further experiments proved that three peptides from H (H9-567, H10-250, and H10-516) and one from F protein (F9-57) were endogenously processed and presented on HLA-A2 molecules. All peptides tested in this study are common to 5 different strains of MV including Edmonston. In both A2Kb and HHD-2 mice, the identified peptide epitopes induced protective immunity against recombinant vaccinia viruses expressing H or F. Because F and H proteins induce neutralizing antibodies, they are major components of new vaccine strategies, and therefore data from this study will contribute to the development of new vaccines against MV infection.
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Item Type: |
Article
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Status: |
Published |
Creators/Authors: |
Creators | Email | Pitt Username | ORCID  |
---|
Oh, SK | | | | Stegman, B | | | | Pendleton, CD | | | | Ota, MO | | | | Pan, CH | | | | Griffin, DE | | | | Burke, DS | donburke@pitt.edu | DONBURKE | | Berzofsky, JA | | | |
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Centers: |
Other Centers, Institutes, Offices, or Units > Center for Vaccine Research |
Date: |
1 September 2006 |
Date Type: |
Publication |
Journal or Publication Title: |
Virology |
Volume: |
352 |
Number: |
2 |
Page Range: |
390 - 399 |
DOI or Unique Handle: |
10.1016/j.virol.2006.04.040 |
Schools and Programs: |
Graduate School of Public Health > Epidemiology |
Refereed: |
Yes |
ISSN: |
0042-6822 |
Date Deposited: |
06 Apr 2015 17:52 |
Last Modified: |
16 Feb 2019 15:55 |
URI: |
http://d-scholarship.pitt.edu/id/eprint/24463 |
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