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Protective immunity provided by HLA-A2 epitopes for fusion and hemagglutinin proteins of measles virus

Oh, SK and Stegman, B and Pendleton, CD and Ota, MO and Pan, CH and Griffin, DE and Burke, DS and Berzofsky, JA (2006) Protective immunity provided by HLA-A2 epitopes for fusion and hemagglutinin proteins of measles virus. Virology, 352 (2). 390 - 399. ISSN 0042-6822

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Abstract

Natural infection and vaccination with a live-attenuated measles virus (MV) induce CD8+ T-cell-mediated immune responses that may play a central role in controlling MV infection. In this study, we show that newly identified human HLA-A2 epitopes from MV hemagglutinin (H) and fusion (F) proteins induced protective immunity in HLA-A2 transgenic mice challenged with recombinant vaccinia viruses expressing F or H protein. HLA-A2 epitopes were predicted and synthesized. Five and four peptides from H and F, respectively, bound to HLA-A2 molecules in a T2-binding assay, and four from H and two from F could induce peptide-specific CD8+ T cell responses in HLA-A2 transgenic mice. Further experiments proved that three peptides from H (H9-567, H10-250, and H10-516) and one from F protein (F9-57) were endogenously processed and presented on HLA-A2 molecules. All peptides tested in this study are common to 5 different strains of MV including Edmonston. In both A2Kb and HHD-2 mice, the identified peptide epitopes induced protective immunity against recombinant vaccinia viruses expressing H or F. Because F and H proteins induce neutralizing antibodies, they are major components of new vaccine strategies, and therefore data from this study will contribute to the development of new vaccines against MV infection.

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Details

Item Type: Article
Status: Published
Creators/Authors:
CreatorsEmailPitt UsernameORCID
Oh, SK
Stegman, B
Pendleton, CD
Ota, MO
Pan, CH
Griffin, DE
Burke, DSdonburke@pitt.eduDONBURKE
Berzofsky, JA
Centers: Other Centers, Institutes, Offices, or Units > Center for Vaccine Research
Date: 1 September 2006
Date Type: Publication
Journal or Publication Title: Virology
Volume: 352
Number: 2
Page Range: 390 - 399
DOI or Unique Handle: 10.1016/j.virol.2006.04.040
Schools and Programs: Graduate School of Public Health > Epidemiology
Refereed: Yes
ISSN: 0042-6822
Date Deposited: 06 Apr 2015 17:52
Last Modified: 16 Feb 2019 15:55
URI: http://d-scholarship.pitt.edu/id/eprint/24463

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