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Validation of the relationship between MRCI polymorphisms with dengue disease in a Brazilian population

Polglase, Hannah (2015) Validation of the relationship between MRCI polymorphisms with dengue disease in a Brazilian population. Master's Thesis, University of Pittsburgh. (Unpublished)

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Abstract

Dengue is a viral disease characterized by mild febrile illness in the majority of cases. In a small percent of cases, it manifests more severe symptoms, and it can be fatal without proper treatment and support. It is a vector borne disease that is transmitted by the Aedes mosquitos. Currently up to 4 billion of the world’s population lives in areas at risk for dengue transmission. This represents a public health risk.
The objective of this study was to evaluate the association of two single nucleotide polymorphisms (SNPs) located in the MRCI gene, rs2296414 and rs34039386, with dengue virus infection in a sample of patients from a cohort study of dengue patients and controls from Recife, Brazil. The MRCI gene codes for the mannose receptors of macrophages, which have been demonstrated to play an important role in viral infections. DNA was amplified using PCR. Genotyping of 179 individuals was done by three methods, fluorescence polarization, restriction digests, and sequencing. All samples were found to be in Hardy-Weinberg equilibrium across the sample population and when classified as cases of controls. No significant departures from Hardy-Weinberg were seen when samples were broken down by disease status. Odds ratios were calculated with novel data and combined with data from a previous study. The previous study examined the same two polymorphisms using a different sample from the same cohort study. A statistically significant difference was found in the combined samples for the rs2296414 site with regards to case or control status, OR=1.46, p=.035. A statistically significant difference was also found for the rs34039386 site with regards to case/control status OR=2.76, p=.0029. This difference was only found in the novel data.
Further research with larger samples sized is needed to continue to understand the relationships between these sites and dengue infection, as the sample size for this study was small. Even when combined with data from the previous study, the data set was not particularly large. The public health significance of this research is produced by the increasing global importance of dengue and the need for more effective treatments and the development of a vaccine; advancement of the understanding of the relationships between the dengue virus and the MRCI gene may lead to improvements in the treatments available and in the production of a vaccine with high efficacy.


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Details

Item Type: University of Pittsburgh ETD
Status: Unpublished
Creators/Authors:
CreatorsEmailPitt UsernameORCID
Polglase, Hannahhapolglase@gmail.com
ETD Committee:
TitleMemberEmail AddressPitt UsernameORCID
Committee ChairMarques, Ernesto T Amarques@pitt.eduMARQUES
Committee MemberMartinson, Jeremy Jjmartins@pitt.eduJMARTINS
Committee MemberFerrell, Robert Erferrell@helix.hgen.pitt.edu
Date: 11 June 2015
Date Type: Publication
Defense Date: 6 April 2015
Approval Date: 11 June 2015
Submission Date: 3 April 2015
Access Restriction: 2 year -- Restrict access to University of Pittsburgh for a period of 2 years.
Number of Pages: 73
Institution: University of Pittsburgh
Schools and Programs: Graduate School of Public Health > Infectious Diseases and Microbiology
Degree: MPH - Master of Public Health
Thesis Type: Master's Thesis
Refereed: Yes
Uncontrolled Keywords: Dengue, MRCI, polymorphisms, SNP
Date Deposited: 11 Jun 2015 16:41
Last Modified: 01 May 2017 05:15
URI: http://d-scholarship.pitt.edu/id/eprint/24484

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