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The use of confidence regions to determine objective criteria for analysis of single parameter histograms of mean fluorescence intensity

Kim, Dongha (2015) The use of confidence regions to determine objective criteria for analysis of single parameter histograms of mean fluorescence intensity. Master's Thesis, University of Pittsburgh. (Unpublished)

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Several methods have been proposed for comparing fluorescence intensity curves obtained from flow cytometry analysis. The one comparison method that is most often used is the Kolmogorov-Smirnov (K-S) test, which is known to find statistically significant differences between curves even when clinical or scientific differences are doubtful. In the field of flow cytometry, the combination of the gating process and either Overton Subtraction or K-S test yields a histogram subtraction technique for analysis of flow cytometric frequency data. In order to help biologists assess the results of a K-S or other test for the difference between mean fluorescence intensity curves, we have proposed a method of producing bootstrap-based confidence bands around the difference in histograms to show the range of intensity channels for which a difference between histograms may be identified. As an illustration of the method, we obtain and display confidence bands for histogram differences between pumping cell subsets. The confidence bands are constructed using both pointwise and simultaneous confidence bands. We compare the results of these two approaches. Furthermore, for descriptive purposes, the confidence bands produced were found to provide a useful descriptive display to aid in the interpretation of results. Flow cytometry is a major research tool for immunology and, by extension, infectious diseases and public health. Our statistical methodology is applicable to flow cytometry research in a variety of settings.


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Item Type: University of Pittsburgh ETD
Status: Unpublished
CreatorsEmailPitt UsernameORCID
Kim, Donghadok27@pitt.eduDOK270000-0002-7423-100X
ETD Committee:
TitleMemberEmail AddressPitt UsernameORCID
Committee ChairWilson, John W.jww@pitt.eduJWW
Committee MemberBandos, Andriyanb61@pitt.eduANB61
Committee MemberDonnenberg, Vera S.donnenbergvs@upmc.eduVESST7
Date: 29 June 2015
Date Type: Publication
Defense Date: 22 April 2015
Approval Date: 29 June 2015
Submission Date: 6 April 2015
Access Restriction: No restriction; Release the ETD for access worldwide immediately.
Number of Pages: 65
Institution: University of Pittsburgh
Schools and Programs: School of Public Health > Biostatistics
Degree: MS - Master of Science
Thesis Type: Master's Thesis
Refereed: Yes
Uncontrolled Keywords: Flow cytometry, Mean Fluorescent Intensity (MFI), Single parameter histogram, Bootstrapping, Difference, Pointwise confidence intervals, Simultaneous confidence bands
Date Deposited: 29 Jun 2015 13:59
Last Modified: 19 Dec 2016 14:42


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