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Cellular heterogeneity in the mouse esophagus implicates the presence of a nonquiescent epithelial stem cell population

DeWard, AD and Cramer, J and Lagasse, E (2014) Cellular heterogeneity in the mouse esophagus implicates the presence of a nonquiescent epithelial stem cell population. Cell Reports, 9 (2). 701 - 711.

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Abstract

© 2014 The Authors. Because the esophageal epithelium lacks a defined stem cell niche, it is unclear whether all basal epithelial cells in the adult esophagus are functionally equivalent. In this study, we showed that basal cells in the mouse esophagus contained a heterogeneous population of epithelial cells, similar to other rapidly cycling tissues such as the intestine or skin. Using a combination of cell-surface markers, we separated primary esophageal tissue into distinct cell populations that harbored differences in stem cell potential. We also used an in vitro 3D organoid assay to demonstrate that Sox2, Wnt, and bone morphogenetic protein signaling regulate esophageal self-renewal. Finally, we labeled proliferating basal epithelial cells in vivo to show differing cell-cycle profiles and proliferation kinetics. Based on our results, we propose that a nonquiescent stem cell population resides in the basal epithelium of the mouse esophagus.


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Details

Item Type: Article
Status: Published
Creators/Authors:
CreatorsEmailPitt UsernameORCID
DeWard, AD
Cramer, J
Lagasse, Elagasse@pitt.eduLAGASSE
Centers: Other Centers, Institutes, or Units > McGowan Institute for Regenerative Medicine
Date: 1 January 2014
Date Type: Publication
Journal or Publication Title: Cell Reports
Volume: 9
Number: 2
Page Range: 701 - 711
DOI or Unique Handle: 10.1016/j.celrep.2014.09.027
Schools and Programs: School of Medicine > Pathology
Refereed: Yes
Date Deposited: 22 May 2015 21:52
Last Modified: 04 Feb 2019 19:55
URI: http://d-scholarship.pitt.edu/id/eprint/24719

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