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Synthesis of chemoreversible prodrugs of ara-C with variable time-release profiles. Biological evaluation of their apoptotic activity

Wipf, P and Li, W and Adeyeye, CM and Rusnak, JM and Lazo, JS (1996) Synthesis of chemoreversible prodrugs of ara-C with variable time-release profiles. Biological evaluation of their apoptotic activity. Bioorganic and Medicinal Chemistry, 4 (10). 1585 - 1596. ISSN 0968-0896

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Abstract

N4-Dipeptidyl slow-release forms of the anticancer drug ara-C were prepared by acylation of the lithiated nucleotide with 4,4-dialkyloxazolinones. An azapeptide prodrug of ara-C was obtained by condensation of an amino acid hydrazide with an activated nucleotide urea. The use of unnatural amino acid residues at N4 prevented nonspecific proteolytic cleavage in biological medium. Ara-C prodrugs 10, 15, 17, and 19 released active drug with half-lives from a few minutes to several days, respectively. Activation via intramolecular N4-deacylation did not require enzymatic intervention but was strictly dependent on the structure of the peptide chain. The prodrugs 10, 15, and 17 produced similar growth inhibition as ara-C in cultured murine leukemia cells while the azapeptide prodrug 19 was less potent reflecting the slow release of active drug with this compound. All four prodrugs retained the ability to induce apoptosis in human HL-60 leukemia cells with kinetics dictated by the rate of intramolecular N4-deacylation. This is the first demonstration for the control of apoptotic cell death by the modulation of drug release from prodrugs.


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Details

Item Type: Article
Status: Published
Creators/Authors:
CreatorsEmailPitt UsernameORCID
Wipf, Ppwipf@pitt.eduPWIPF
Li, W
Adeyeye, CM
Rusnak, JM
Lazo, JS
Date: 1 January 1996
Date Type: Publication
Journal or Publication Title: Bioorganic and Medicinal Chemistry
Volume: 4
Number: 10
Page Range: 1585 - 1596
DOI or Unique Handle: 10.1016/0968-0896(96)00153-8
Schools and Programs: Dietrich School of Arts and Sciences > Chemistry
Refereed: Yes
ISSN: 0968-0896
Date Deposited: 22 Jul 2015 13:13
Last Modified: 02 Feb 2019 15:56
URI: http://d-scholarship.pitt.edu/id/eprint/25646

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