Lawrence, Chad
(2015)
ATM copy number loss as a biomarker for radioresistance in urothelial bladder cancer cell lines.
Master's Thesis, University of Pittsburgh.
(Unpublished)
Abstract
Cancer is one of the leading causes of death in the United States today. Various techniques have been adopted to combat this disease. These techniques include chemotherapy and ionizing radiation therapy. Previous studies have suggested that certain cancer cells are more likely to survive when exposed to ionizing radiation, a phenomenon known as radioresistance. In oral squamous cell carcinoma, one biomarker for radioresistance is the copy number loss of distal 11q (marked by the ATM gene) and defective TP53 function. Our study is designed to determine whether number loss of ATM is linked with radioresistance instead of the combination of distal 11q and defective TP53 function. Based on clonogenic data, we hypothesize that other factors including miR100 and/or SMARCA5 should be investigated as potential predictors of radioresistance.
Public Health Significance: Cancer is a widespread public health problem. The goal of this research is to identify targets that lead to increased therapeutic efficacy. In light of this new evidence, further studies can be designed to establish a more accurate biomarker for radioresistance in urothelial bladder cancer cell lines. Further studies should investigate the roles of miR100 and SMARCA5 in radioresistance in these cell lines. In essence, this research study shows that the ATM gene may not be the best predictor of radioresistance in bladder cancer cell lines, but perhaps a combination of events can explain our findings.
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Details
| Item Type: |
University of Pittsburgh ETD
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| Status: |
Unpublished |
| Creators/Authors: |
|
| ETD Committee: |
| Title | Member | Email Address | Pitt Username | ORCID |
|---|
| Thesis Advisor | Gollin, Susanne | | | | | Committee Member | Parikh, Rahul | | | | | Committee Member | Barmada, Michael | | | |
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| Date: |
28 September 2015 |
| Date Type: |
Publication |
| Defense Date: |
15 July 2015 |
| Approval Date: |
28 September 2015 |
| Submission Date: |
17 July 2015 |
| Access Restriction: |
3 year -- Restrict access to University of Pittsburgh for a period of 3 years. |
| Number of Pages: |
58 |
| Institution: |
University of Pittsburgh |
| Schools and Programs: |
School of Public Health > Human Genetics |
| Degree: |
MS - Master of Science |
| Thesis Type: |
Master's Thesis |
| Refereed: |
Yes |
| Uncontrolled Keywords: |
Bladder Cancer ionizing radiation resistance atm ataxia telangectasia mutated biomarker |
| Date Deposited: |
28 Sep 2015 18:31 |
| Last Modified: |
01 Sep 2018 05:15 |
| URI: |
http://d-scholarship.pitt.edu/id/eprint/25666 |
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