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Mobilization of the non-conjugative plasmid RSF1010: A genetic and DNA sequence analysis of the mobilization region

Derbyshire, KM and Hatfull, G and Willetts, N (1987) Mobilization of the non-conjugative plasmid RSF1010: A genetic and DNA sequence analysis of the mobilization region. MGG Molecular & General Genetics, 206 (1). 161 - 168. ISSN 0026-8925

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Abstract

The entire region required for mobilization of the non-conjugative plasmid RSF1010 has been cloned into a mobilization-deficient pBR322 derivative. The segment of DNA cloned was ∼1.8 kb and included the origin of conjugal DNA transfer (oriT). The DNA sequence of the mobilization region has been determined, and revealed the presence of several overlapping reading frames. The isolation and mapping of both Tn 1725 and BamH1-linker insertions and comparison with the DNA sequence data has allowed the identification of three genes required for mobilization. Two of these genes are overlapping and encode proteins of 16 kDa and >65 kDa (although the truncated protein is functional, the gene extends outside the region cloned). The third gene is transcribed in the opposite direction. Promoters capable of transcribing these genes were located by S1 mapping in the inter-cistronic region between these divergently transcribed genes. The oriT site is located in this region, and the transcriptional patterns observed for mob+ and mob- plasmids implied that the promoters may be regulated by two of the mobilization proteins binding to the oriT site. © 1987 Springer-Verlag.


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Details

Item Type: Article
Status: Published
Creators/Authors:
CreatorsEmailPitt UsernameORCID
Derbyshire, KM
Hatfull, Ggfh@pitt.eduGFH
Willetts, N
Date: 1 January 1987
Date Type: Publication
Journal or Publication Title: MGG Molecular & General Genetics
Volume: 206
Number: 1
Page Range: 161 - 168
DOI or Unique Handle: 10.1007/bf00326552
Schools and Programs: Dietrich School of Arts and Sciences > Biological Sciences
Refereed: Yes
ISSN: 0026-8925
MeSH Headings: Base Sequence; Cloning, Molecular; DNA Restriction Enzymes; Endonucleases; Escherichia coli--genetics; Genetic Complementation Test; Plasmids; Promoter Regions, Genetic; Single-Strand Specific DNA and RNA Endonucleases; Transcription, Genetic
PubMed ID: 3033438
Date Deposited: 30 Oct 2015 15:04
Last Modified: 20 Jul 2019 14:55
URI: http://d-scholarship.pitt.edu/id/eprint/26244

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