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Deletion of the spf (spot 42 RNA) gene of Escherichia coli

Hatfull, GF and Joyce, CM (1986) Deletion of the spf (spot 42 RNA) gene of Escherichia coli. Journal of Bacteriology, 166 (3). 746 - 750. ISSN 0021-9193

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To investigate the function of spot 42 RNA, a small RNA of Escherichia coli, we constructed a strain in which spf, the structural gene for this RNA, is deleted. We achieved this by using a Δatt phage λ carrying a DNA fragment spanning the spf region but with a precise deletion of spf. By integration of this phage at the spf locus and by its subsequent excision, we were able to cross the spf deletion onto the bacterial chromosome. The fact that such a deletion could be obtained indicated that spf is not an essential gene. We did not observe any major defect in Aspf cells, although in one strain background the deletion caused a slight growth impairment.


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Item Type: Article
Status: Published
CreatorsEmailPitt UsernameORCID
Hatfull, GFgfh@pitt.eduGFH
Joyce, CM
Date: 1 January 1986
Date Type: Publication
Journal or Publication Title: Journal of Bacteriology
Volume: 166
Number: 3
Page Range: 746 - 750
DOI or Unique Handle: 10.1128/jb.166.3.746-750.1986
Schools and Programs: Dietrich School of Arts and Sciences > Biological Sciences
Refereed: Yes
ISSN: 0021-9193
MeSH Headings: Bacteriophage lambda--genetics; Chromosome Deletion; Escherichia coli--genetics; Genes; Genes, Bacterial; Lysogeny; Nucleic Acid Hybridization; Phenotype; RNA, Small Nuclear--genetics
Other ID: NLM PMC215189
PubMed Central ID: PMC215189
PubMed ID: 2940230
Date Deposited: 23 Nov 2015 15:20
Last Modified: 02 Feb 2019 15:57


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