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Comparison of nasopharyngeal and mid-turbinate swabs in children with upper respiratory tract infection symptoms and the feasibility of using electronic diaries in clinical research

Simonian, Anita (2016) Comparison of nasopharyngeal and mid-turbinate swabs in children with upper respiratory tract infection symptoms and the feasibility of using electronic diaries in clinical research. Master's Thesis, University of Pittsburgh. (Unpublished)

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Background: Acute bacterial sinusitis is the inflammation of the sinuses resulting from a bacterial infection. Sinusitis is the fifth most common condition for which antimicrobials are prescribed to children in the United States. According to the literature, 40% of children diagnosed with sinusitis do not have a true bacterial infection. The development of antibiotic resistance has become a significant public health concern among this pediatric population.
Methods: Participants between the ages of 6 months and 11 years were recruited from Children’s Hospital of Pittsburgh (CHP) and the Primary Care Center in Oakland of CHP. Two studies, the Cold Swab study and the Sinus Diary study were designed to investigate acute bacterial sinusitis in children. Nasopharyngeal (NP) and mid-turbinate (MT) swabs were obtained from children presenting with upper respiratory tract infection (URI) symptoms enrolled in the Cold Swab study. NP and MT swab results were compared in order to determine success of colonization. All laboratory work was conducted at the Infectious Disease Research Laboratory of CHP. The Sinus Diary study investigated the feasibility of having parents electronically report their child’s URI symptoms daily.
Results: The Cold Swab study enrolled 96 children and the Sinus Diary study enrolled 68 participants. Based on the results of the Cold Swab study, 9.4% of children were colonized with both SPN and HFLU, 48% were colonized with SPN only, 25% were colonized with HFLU only, and 36.4% were not colonized with either pathogen. Seventy-six percent of paired NP/MT swabs were concordant for SPN and HFLU, and 87.5% of paired swabs were concordant for only SPN or only HFLU. The compliance of the electronic diary study was assessed. Of parents who successfully completed all of the diary entries, only 50% did not require a reminder phone call.
Conclusion: The NP/MT swabs were highly concordant for SPN and HFLU in the Cold Swab study. Electronic diaries are an effective way of collecting data for clinical research, however reminders are essential for achieving a high compliance rate. The results from these pilot studies will be used as preliminary data for a larger acute bacterial sinusitis study.


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Item Type: University of Pittsburgh ETD
Status: Unpublished
CreatorsEmailPitt UsernameORCID
Simonian, Anitaans243@pitt.eduANS243
ETD Committee:
TitleMemberEmail AddressPitt UsernameORCID
Committee ChairMartin, Judith Mjudy.martin@chp.eduMARTINJU
Committee MemberMartinson, Jeremy Jjmartins@pitt.eduJMARTINS
Committee MemberHaggerty, Catherinehaggerty@pitt.eduHAGGERTY
Date: 27 January 2016
Date Type: Publication
Defense Date: 3 December 2015
Approval Date: 27 January 2016
Submission Date: 23 November 2015
Access Restriction: 2 year -- Restrict access to University of Pittsburgh for a period of 2 years.
Number of Pages: 46
Institution: University of Pittsburgh
Schools and Programs: School of Public Health > Infectious Diseases and Microbiology
Degree: MPH - Master of Public Health
Thesis Type: Master's Thesis
Refereed: Yes
Uncontrolled Keywords: Sinusitis, Bacterial, Pediatrics, Microbiology, Electronic Diaries
Date Deposited: 27 Jan 2016 21:04
Last Modified: 01 Jan 2018 06:15


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