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Cholecystokinin activates catecholaminergic neurons in the caudal medulla that innervate the paraventricular nucleus of the hypothalamus in rats

Rinaman, L and Hoffman, GE and Dohanics, J and Le, W and Stricker, EM and Verbalis, JG (1995) Cholecystokinin activates catecholaminergic neurons in the caudal medulla that innervate the paraventricular nucleus of the hypothalamus in rats. Journal of Comparative Neurology, 360 (2). 246 - 256. ISSN 0021-9967

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Abstract

Stimulation of gastric vagal afferents by systemic administration of cholecystokinin octapeptide (CCK) inhibits gastric motility, reduces food intake, and stimulates pituitary secretion of oxytocin and adrenocorticotropic hormone in rats. To characterize further the central neurol circuits responsible for these effects, the present study used triple‐labeling immunocytochemical methods to determine whether or not exogenous CCK activates cFos expression in catecholaminergic neurons in the caudal medulla that project to the paraventricular nucleus of the hypothalamus (PVN). To identify these neurons, the retrograde tracer fluorogold (FG) was iontophoresed into the PVN of anesthetized rats under stereotaxic guidance. After 2 weeks, rats were injected with CCK (100 μg/kg, i. p.) and then anesthetized and killed 1 hour later by perfusion fixation. Medullary sections were processed for triple immunocytochemical localization of cFos, retrogradely transported FG, and tyrosine hydroxylase (TH). In rats with FG injections centered in the PVN (n = 10), approximately 70% of the FG‐labeled neurons in the caudal nucleus of the solitary tract (NST) and ventrolateral medulla (VLM) expressed cFos. Of these activated PVN‐projecting neurons, approximately 78% in the NST and 89% in the VLM were catecholaminergic (TH positive). These results indicate that PVN‐projecting catecholaminergic neurons within the caudal medulla are activated by periph eral administration of CCK, further implicating these ascending catecholaminergic path ways in the neuroendocrine, physiological, and behavioral effects produced by gastric vagal stimulation. © 1995 Wiley‐Liss, Inc. Copyright © 1995 Wiley‐Liss, Inc.


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Details

Item Type: Article
Status: Published
Creators/Authors:
CreatorsEmailPitt UsernameORCID
Rinaman, Lrinaman@pitt.eduRINAMAN
Hoffman, GE
Dohanics, J
Le, W
Stricker, EM
Verbalis, JG
Date: 1 January 1995
Date Type: Publication
Journal or Publication Title: Journal of Comparative Neurology
Volume: 360
Number: 2
Page Range: 246 - 256
DOI or Unique Handle: 10.1002/cne.903600204
Refereed: Yes
ISSN: 0021-9967
MeSH Headings: Afferent Pathways--drug effects; Animals; Catecholamines--metabolism; Cholecystokinin--pharmacology; Eating--drug effects; Hypothalamus--drug effects; Male; Neurons--drug effects; Paraventricular Hypothalamic Nucleus--drug effects; Rats; Rats, Sprague-Dawley; Stomach--drug effects
PubMed ID: 8522645
Date Deposited: 25 Feb 2016 17:36
Last Modified: 02 Feb 2019 16:57
URI: http://d-scholarship.pitt.edu/id/eprint/26843

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