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Progressive postnatal assembly of limbic-autonomic circuits revealed by central transneuronal transport of pseudorabies virus

Rinaman, L and Levitt, P and Card, JP (2000) Progressive postnatal assembly of limbic-autonomic circuits revealed by central transneuronal transport of pseudorabies virus. Journal of Neuroscience, 20 (7). 2731 - 2741. ISSN 0270-6474

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Abstract

The development of neuronal projections to a target and the establishment of synaptic connections with that target can be temporally distinct events, which typically are distinguished by functional assessments. We have applied a novel neuroanatomical approach to characterize the development of limbic forebrain synaptic inputs to autonomic neurons in neonatal rats. Transneuronal labeling of preautonomic forebrain neurons was achieved by inoculating the ventral stomach wall with pseudorabies virus (PRV) on postnatal day 1 (P1), P4, or P8. In each age group, PRV-positive neurons were present in autonomic and preautonomic regions of the spinal cord and brainstem 62-64 hr after inoculation. Transneuronal forebrain labeling in rats injected on P8 was similar to the transneuronal labeling reported previously in adult rats and included neurons in the medial and lateral hypothalamus, amygdala, bed nucleus of the stria terminalis, and visceral cortices. However, no cortex labeling and only modest amygdala and bed nucleus labeling were observed in rats injected with PRV on P4, and only medial hypothalamic labeling was observed in rats injected on P1. Additional tracing experiments involving central injections of PRV or cholera toxin β indicated that lateral hypothalamic and telencephalic regions projected to the medullary dorsal vagal complex several days before establishing synaptic connections with gastric-related autonomic neurons. These results demonstrate a novel strategy for evaluating synaptic connectivity in developing neural circuits and show a temporally segregated postnatal emergence of medial hypothalamic, lateral hypothalamic, and telencephalic synaptic inputs to central autonomic neurons.

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Details

Item Type: Article
Status: Published
Creators/Authors:
CreatorsEmailPitt UsernameORCID
Rinaman, Lrinaman@pitt.eduRINAMAN
Levitt, P
Card, JP
Date: 1 April 2000
Date Type: Publication
Journal or Publication Title: Journal of Neuroscience
Volume: 20
Number: 7
Page Range: 2731 - 2741
DOI or Unique Handle: 10.1523/jneurosci.20-07-02731.2000
Refereed: Yes
ISSN: 0270-6474
MeSH Headings: Aging; Amygdala--growth & development; Animals; Animals, Newborn--growth & development; Autonomic Nervous System--growth & development; Autonomic Nervous System--virology; Axonal Transport; Cerebral Cortex--growth & development; Herpesvirus 1, Suid; Limbic System--growth & development; Limbic System--virology; Neurons--virology; Paraventricular Hypothalamic Nucleus--growth & development; Rats; Stomach--virology
PubMed ID: 10729354
Date Deposited: 02 Mar 2016 14:56
Last Modified: 20 Jun 2020 22:55
URI: http://d-scholarship.pitt.edu/id/eprint/26883

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