Link to the University of Pittsburgh Homepage
Link to the University Library System Homepage Link to the Contact Us Form
D-Scholarship @ Pitt Banner and Links to Homepage

Physiologic compliance in engineered small-diameter arterial constructs based on an elastomeric substrate.

Crapo, Peter M and Wang, Yadong (2009) Physiologic compliance in engineered small-diameter arterial constructs based on an elastomeric substrate. Biomaterials, 31 (7). 1626 - 1635.

[img]
Preview
 PDF (biodegradable elastomers and tissue engineered blood vessels)
Published Version
Available under License : See the attached license file.
Download (2MB) | Preview
[img] Plain Text (licence)
Available under License : See the attached license file.
Download (1kB)

Abstract

Compliance mismatch is a significant challenge to long-term patency in small-diameter bypass grafts because it causes intimal hyperplasia and ultimately graft occlusion. Current engineered grafts are typically stiff with high burst pressure but low compliance and low elastin expression. We postulated that engineering small arteries on elastomeric scaffolds under dynamic mechanical stimulation would result in strong and compliant arterial constructs. This study compares properties of engineered arterial constructs based on biodegradable polyester scaffolds composed of either rigid poly(lactide-co-glycolide) (PLGA) or elastomeric poly(glycerol sebacate) (PGS). Adult baboon arterial smooth muscle cells (SMCs) were cultured in vitro for 10 days in tubular, porous scaffolds. Scaffolds were significantly stronger after culture regardless of material, but the elastic modulus of PLGA constructs was an order of magnitude greater than that of porcine carotid arteries and PGS constructs. Deformation was elastic in PGS constructs and carotid arteries but plastic in PLGA constructs. Compliance of arteries and PGS constructs were equivalent at pressures tested. Altering scaffold material from PLGA to PGS significantly decreased collagen content and significantly increased insoluble elastin content in constructs without affecting soluble elastin concentration in the culture medium. PLGA constructs contained no appreciable insoluble elastin. This research demonstrates that: (1) substrate stiffness directly affects in vitro tissue development and mechanical properties; (2) rigid materials likely inhibit elastin incorporation into the extracellular matrix of engineered arterial tissues; and (3) grafts with physiologic compliance and significant elastin content can be engineered in vitro after only days of cell culture.

Share

Citation/Export:
Social Networking:
Share |

Details

Item Type: Article
Status: Published
Creators/Authors:
CreatorsEmailPitt UsernameORCID
Crapo, Peter M
Wang, Yadongyaw20@pitt.eduYAW20orcid.org/0000-0003-2067-382X
Date: 14 November 2009
Date Type: Acceptance
Journal or Publication Title: Biomaterials
Volume: 31
Number: 7
Page Range: 1626 - 1635
DOI or Unique Handle: 10.1016/j.biomaterials.2009.11.035
Institution: University of Pittsburgh
Schools and Programs: Swanson School of Engineering > Bioengineering
Refereed: Yes
Uncontrolled Keywords: Animals, Arteries, Cell Shape, Collagen, Compliance, Decanoates, Elastic Modulus, Elastin, Elastomers, Glycerol, Male, Materials Testing, Microscopy, Electron, Papio, Polymers, Tissue Engineering, Tissue Scaffolds
Funders: NHLBI NIH HHS (R01 HL089658), NHLBI NIH HHS (R01 HL089658-02), NHLBI NIH HHS (R01HL089658-01)
Date Deposited: 13 Jan 2010 18:14
Last Modified: 01 Aug 2020 19:55
URI: http://d-scholarship.pitt.edu/id/eprint/2798

Metrics

Monthly Views for the past 3 years

Plum Analytics

Altmetric.com

Actions (login required)

View Item View Item