Sanyal, Anwesha
(2016)
Identification of cellular factors involved in Neisseria gonorrhoea induced enhanced HIV-1 transmission in a cervical tissue based organ culture model.
Doctoral Dissertation, University of Pittsburgh.
(Unpublished)
Abstract
The presence of sexually transmitted infections (STI) such as Neisseria gonorrhoeae (NG) can enhance the transmission of HIV-1. Our goal in this study is to elucidate mechanism by which NG induces enhanced HIV-1 transmission. A cervical tissue based organ culture system was developed to study the interaction between NG and HIV-1 which provided a unique opportunity to elucidate mechanism of NG induced enhanced HIV-1 transmission across cervical mucosa. We demonstrated that the NG exposure on the cervical tissue in the organ culture model showed physical characteristics of NG infection and induced high levels of inflammatory cytokines IL1-β and TNF-α that have been observed during in-vivo NG infection in the cervix. In elucidating the mechanism of NG induced enhancement of HIV-1 transmission, we demonstrated that NG not by itself but the culture fluids from NG exposed tissues (reminiscent of cervical milieu) increased HIV-1 transcription from the HIV- LTR in TZM-bl cells, induced full length HIV-1 from latently infected U1 and ACH2 cells and increased transmission of HIV-1 across cervical mucosa. The whole genome transcriptome analysis using second-generation sequencing of the micro-dissected epithelial layer of the tissues exposed to NG and HIV-1 identified with high statistical significance differentially expressed genes in NG exposed and HIV-1 exposed tissues, and identified common cellular factors as well as pathways involved in cell activation, migration and stimulation expressed in the epithelia.
Out of these only two differentially expressed genes that were common between tissues exposed to both NG and HIV-1 are CXCL10 and IL8. Addition of inhibitors of CXCL10 and IL8 suppressed HIV-1 transmission, while addition of CXCL10 and IL8 increased HIV-1 transmission indicating that CXCL10 and IL8 could be involved in HIV-1 transmission across cervical epithelia. IL-1β also increased CXCL10 and IL8 expression in cervical tissues and enhanced HIV-1 transmission Altogether these data are consistent with a model (Figure 42) for NG induced enhanced HIV-1 transmission: NG infection secretes IL-1β which induces increased production of epithelial CXCL10 and IL-8 causing the migration of HIV-1 target cells CD3+T cells and macrophages towards intraepithelial region that fuels HIV-1 replication in submucosa and consequently enhances HIV-1 transmission.
Taken together, our results confirm that the risk of acquisition of HIV-1 infection in the ecto-cervical region increases with prior NG infection. From the public health perspective, identification of IL-1β and its target cellular proteins in NG induced enhanced HIV-1 transmission could be useful in development of drugs that impair HIV transmission. Further work in nonhuman primates or humanized mouse models could provide confirmation of the role of CXCL10 and IL-8 in HIV transmission and its modulation by NG secreted proteins like IL-1β in vivo.
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Details
| Item Type: |
University of Pittsburgh ETD
|
| Status: |
Unpublished |
| Creators/Authors: |
|
| ETD Committee: |
|
| Date: |
9 September 2016 |
| Date Type: |
Publication |
| Defense Date: |
13 June 2016 |
| Approval Date: |
9 September 2016 |
| Submission Date: |
28 May 2016 |
| Access Restriction: |
2 year -- Restrict access to University of Pittsburgh for a period of 2 years. |
| Number of Pages: |
169 |
| Institution: |
University of Pittsburgh |
| Schools and Programs: |
School of Public Health > Infectious Diseases and Microbiology |
| Degree: |
PhD - Doctor of Philosophy |
| Thesis Type: |
Doctoral Dissertation |
| Refereed: |
Yes |
| Uncontrolled Keywords: |
NG, HIV-1 TRANSMISSION, CYTOKINES, MUCOSA, EPITHELIUM |
| Date Deposited: |
09 Sep 2016 19:12 |
| Last Modified: |
01 Jul 2018 05:15 |
| URI: |
http://d-scholarship.pitt.edu/id/eprint/28095 |
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