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Low Soluble Syndecan-1 Precedes Preeclampsia

Gandley, RE and Althouse, A and Jeyabalan, A and Bregand-White, JM and McGonigal, S and Myerski, AC and Gallaher, M and Powers, RW and Hubel, CA (2016) Low Soluble Syndecan-1 Precedes Preeclampsia. PLoS ONE, 11 (6). 1 - 18.

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Abstract

© 2016 Gandley et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Introduction Syndecan-1 (Sdc1; CD138) is a major transmembrane heparan sulfate proteoglycan expressed on the extracellular, luminal surface of epithelial cells and syncytiotrophoblast, thus comprising a major component of the glycocalyx of these cells. The "soluble" (shed) form of Sdc1 has paracrine and autocrine functions and is normally produced in a regulated fashion. We compared plasma soluble Sdc1 concentrations, in relation to placental Sdc1 expression, in uncomplicated (control) and preeclamptic pregnancies. Methods We evaluated soluble Sdc1 across uncomplicated pregnancy, and between preeclamptic, gestational hypertensive and control patients at mid-pregnancy (20 weeks) and 3rd trimester by ELISA. Placental expression level of Sdc1 was compared between groups in relation to pre-delivery plasma soluble Sdc1. Participants were recruited from Magee-Womens Hospital. Results In uncomplicated pregnancy, plasma soluble Sdc1 rose significantly in the 1st trimester, and reached an approximate 50-fold increase at term compared to post pregnancy levels. Soluble Sdc1 was lower at mid-pregnancy in women who later developed preeclampsia (P<0.05), but not gestational hypertension, compared to controls, and remained lower at late pregnancy in preeclampsia (P<0.01) compared to controls. Sdc1 was prominently expressed on syncytiotrophoblast of microvilli. Syncytiotrophoblast Sdc1 immunostaining intensities, and mRNA content in villous homogenates, were lower in preeclampsia vs. controls (P<0.05). Soluble Sdc1 and Sdc1 immunostaining scores were inversely associated with systolic blood pressures, and positively correlated with infant birth weight percentile.


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Details

Item Type: Article
Status: Published
Creators/Authors:
CreatorsEmailPitt UsernameORCID
Gandley, REgandleyr@pitt.eduGANDLEYR
Althouse, A
Jeyabalan, Aarunj@pitt.eduARUNJ
Bregand-White, JM
McGonigal, S
Myerski, AC
Gallaher, M
Powers, RWpowersr@pitt.eduPOWERSR
Hubel, CAhubelc@pitt.eduHUBELC
Contributors:
ContributionContributors NameEmailPitt UsernameORCID
EditorZenclussen, Ana ClaudiaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Centers: Other Centers, Institutes, or Units > Magee-Women's Research Institute
Date: 1 June 2016
Date Type: Publication
Access Restriction: No restriction; Release the ETD for access worldwide immediately.
Journal or Publication Title: PLoS ONE
Volume: 11
Number: 6
Page Range: 1 - 18
DOI or Unique Handle: 10.1371/journal.pone.0157608
Institution: University of Pittsburgh
Schools and Programs: School of Medicine > Obstetrics, Gynecology, and Reproductive Sciences
Refereed: Yes
Date Deposited: 16 Jun 2016 15:43
Last Modified: 02 Feb 2019 20:55
URI: http://d-scholarship.pitt.edu/id/eprint/28219

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