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Evaluation of the gastrointestinal tract as potential route of primary polyomavirus infection in mice

Huang, G and Zeng, G and Huang, Y and Ramaswami, B and Randhawa, P (2016) Evaluation of the gastrointestinal tract as potential route of primary polyomavirus infection in mice. PLoS ONE, 11 (3).

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Abstract

Background Detection of Polyomavirus (PyV) DNA in metropolitan rivers worldwide has led to the suggestion that primary viral infection can occur by the oral route. The aim of this study was to test this notion experimentally. Methods Mouse PyV (MPyV) was used to infect C57BL/6J mice by the nasal or intragastric route. Viral load kinetics was studied 3, 7, 10, 14, 21 and 28 days post-infection (dpi) using quantitative PCR. Results Following nasal infection, MPyV DNA was readily detected in many organs including lung, heart, aorta, colon, and stool with viral loads in the range of 103-106 copies/mg wet weight that peaked 7-10 dpi. Complete viral clearance occurred in the serum and kidney by 28 dpi, while clearance in other organs was partial with a 10-100 fold decrease in viral load. In contrast, following intragastric infection peak detection of PyV was delayed to 21 dpi, and viral loads were up to 3 logs lower. There was no detectable virus in the heart, colon, or stool. Conclusions The intragastric route of MPyV infection is successful, not as efficacious as the respiratory route, and associated with delayed viral dissemination as well as a lower peak MPyV load in individual organs.

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Details

Item Type: Article
Status: Published
Creators/Authors:
CreatorsEmailPitt UsernameORCID
Huang, G
Zeng, Ggaz3@pitt.eduGAZ3
Huang, Yyuh11@pitt.eduYUH11
Ramaswami, Bbar23@pitt.eduBAR23
Randhawa, Ppsr@pitt.eduPSR
Contributors:
ContributionContributors NameEmailPitt UsernameORCID
EditorSchang, Luis M.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Date: 1 March 2016
Date Type: Publication
Access Restriction: No restriction; Release the ETD for access worldwide immediately.
Journal or Publication Title: PLoS ONE
Volume: 11
Number: 3
DOI or Unique Handle: 10.1371/journal.pone.0150786
Institution: University of Pittsburgh
Schools and Programs: School of Medicine > Pathology
School of Medicine > Surgery
Refereed: Yes
Date Deposited: 23 Aug 2016 15:09
Last Modified: 13 Sep 2023 19:55
URI: http://d-scholarship.pitt.edu/id/eprint/28299

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