Link to the University of Pittsburgh Homepage
Link to the University Library System Homepage Link to the Contact Us Form
D-Scholarship @ Pitt Banner and Links to Homepage

G-protein α-subunit Gsα is required for craniofacial morphogenesis

Lei, R and Zhang, K and Wei, Y and Chen, M and Weinstein, LS and Hong, Y and Zhu, M and Li, H and Li, H (2016) G-protein α-subunit Gsα is required for craniofacial morphogenesis. PLoS ONE, 11 (2).

[img]
Preview
 PDF
Published Version
Available under License : See the attached license file.
Download (22MB)
[img] Plain Text (licence)
Available under License : See the attached license file.
Download (1kB)

Abstract

The heterotrimeric G protein subunit Gsα couples receptors to activate adenylyl cyclase and is required for the intracellular cAMP response and protein kinase A (PKA) activation. Gsα is ubiquitously expressed in many cell types; however, the role of Gsα in neural crest cells (NCCs) remains unclear. Here we report that NCCs-specific Gsα knockout mice die within hours after birth and exhibit dramatic craniofacial malformations, including hypoplastic maxilla and mandible, cleft palate and craniofacial skeleton defects. Histological and anatomical analysis reveal that the cleft palate in Gsα knockout mice is a secondary defect resulting from craniofacial skeleton deficiencies. In Gsα knockout mice, the morphologies of NCCs-derived cranial nerves are normal, but the development of dorsal root and sympathetic ganglia are impaired. Furthermore, loss of Gsα in NCCs does not affect cranial NCCs migration or cell proliferation, but significantly accelerate osteochondrogenic differentiation. Taken together, our study suggests that Gsα is required for neural crest cells-derived craniofacial development.

Share

Citation/Export:
Social Networking:
Share |

Details

Item Type: Article
Status: Published
Creators/Authors:
CreatorsEmailPitt UsernameORCID
Lei, R
Zhang, Kkez11@pitt.eduKEZ11
Wei, Y
Chen, M
Weinstein, LS
Hong, Yyhong@pitt.eduYHONG
Zhu, M
Li, H
Li, H
Contributors:
ContributionContributors NameEmailPitt UsernameORCID
EditorCray, JamesUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Date: 1 February 2016
Date Type: Publication
Access Restriction: No restriction; Release the ETD for access worldwide immediately.
Journal or Publication Title: PLoS ONE
Volume: 11
Number: 2
DOI or Unique Handle: 10.1371/journal.pone.0147535
Institution: University of Pittsburgh
Schools and Programs: School of Medicine > Cell Biology and Molecular Physiology
Refereed: Yes
Date Deposited: 23 Aug 2016 15:07
Last Modified: 30 Mar 2021 13:56
URI: http://d-scholarship.pitt.edu/id/eprint/28312

Metrics

Monthly Views for the past 3 years

Plum Analytics

Altmetric.com

Actions (login required)

View Item View Item