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Comparative proteomics reveals important viral-host interactions in HCV-infected human liver cells

Liu, S and Zhao, T and Song, BB and Zhou, J and Wang, TT (2016) Comparative proteomics reveals important viral-host interactions in HCV-infected human liver cells. PLoS ONE, 11 (1).

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Abstract

© Liu 2016 et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Hepatitis C virus (HCV) poses a global threat to public health. HCV envelop protein E2 is the major component on the virus envelope, which plays an important role in virus entry and morphogenesis. Here, for the first time, we affinity purified E2 complex formed in HCV-infected human hepatoma cells and conducted comparative mass spectrometric analyses. 85 cellular proteins and three viral proteins were successfully identified in three independent trials, among which alphafetoprotein (AFP), UDP-glucose: glycoprotein glucosyltransferase 1 (UGT1) and HCV NS4B were further validated as novel E2 binding partners. Subsequent functional characterization demonstrated that gene silencing of UGT1 in human hepatoma cell line Huh7.5.1 markedly decreased the production of infectious HCV, indicating a regulatory role of UGT1 in viral lifecycle. Domain mapping experiments showed that HCV E2-NS4B interaction requires the transmembrane domains of the two proteins. Altogether, our proteomics study has uncovered key viral and cellular factors that interact with E2 and provided new insights into our understanding of HCV infection.


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Details

Item Type: Article
Status: Published
Creators/Authors:
CreatorsEmailPitt UsernameORCID
Liu, S
Zhao, T
Song, BB
Zhou, Jzho@pitt.eduZHO
Wang, TT
Contributors:
ContributionContributors NameEmailPitt UsernameORCID
EditorPolyak, Stephen J.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Date: 1 January 2016
Date Type: Publication
Access Restriction: No restriction; Release the ETD for access worldwide immediately.
Journal or Publication Title: PLoS ONE
Volume: 11
Number: 1
DOI or Unique Handle: 10.1371/journal.pone.0147991
Institution: University of Pittsburgh
Schools and Programs: School of Medicine > Urology
Refereed: Yes
Date Deposited: 23 Aug 2016 14:56
Last Modified: 04 Feb 2019 23:55
URI: http://d-scholarship.pitt.edu/id/eprint/28317

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