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Aquaporin 5 interacts with fluoride and possibly protects against caries

Anjomshoaa, I and Briseño-Ruiz, J and Deeley, K and Poletta, FA and Mereb, JC and Leite, AL and Barreta, PATM and Silva, TL and Dizak, P and Ruff, T and Patir, A and Koruyucu, M and Abbasoglu, Z and Casado, PL and Brown, A and Zaky, SH and Bayram, M and Küchler, EC and Cooper, ME and Liu, K and Marazita, ML and Tanboga, I and Granjeiro, JM and Seymen, F and Castilla, EE and Orioli, IM and Sfeir, C and Owyang, H and Buzalaf, MAR and Vieira, AR (2015) Aquaporin 5 interacts with fluoride and possibly protects against caries. PLoS ONE, 10 (12).

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Aquaporins (AQP) are water channel proteins and the genes coding for AQP2, AQP5, and AQP6 are clustered in 12q13. Since AQP5 is expressed in serous acinar cells of salivary glands, we investigated its involvement in caries. DNA samples from 1,383 individuals from six groups were studied. Genotypes of eight single nucleotide polymorphisms covering the aquaporin locus were tested for association with caries experience. Interaction with genes involved in enamel formation was tested. The association between enamel microhardness at baseline, after creation of artificial caries lesion, and after exposure to fluoride and the genetic markers in AQP5 was tested. Finally, AQP5 expression in human whole saliva, after exposure to fluoride in a mammary gland cell line, which is known to express AQP5, and in Wistar rats was also verified. Nominal associations were found between caries experience and markers in the AQP5 locus. Since these associations suggested that AQP5 may be inhibited by levels of fluoride in the drinking water that cause fluorosis, we showed that fluoride levels above optimal levels change AQP5 expression in humans, cell lines, and rats. We have shown that AQP5 is involved in the pathogenesis of caries and likely interacts with fluoride.


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Item Type: Article
Status: Published
CreatorsEmailPitt UsernameORCID
Anjomshoaa, I
Briseño-Ruiz, J
Deeley, Kkbd1@pitt.eduKBD1
Poletta, FA
Mereb, JC
Leite, AL
Barreta, PATM
Silva, TL
Dizak, Ppmd29@pitt.eduPMD29
Ruff, T
Patir, A
Koruyucu, M
Abbasoglu, Z
Casado, PL
Brown, Aajb68@pitt.eduAJB680000-0002-8669-3439
Zaky, SH
Bayram, M
Küchler, EC
Cooper, ME
Liu, K
Marazita, MLmarazita@pitt.eduMARAZITA
Tanboga, I
Granjeiro, JM
Seymen, F
Castilla, EE
Orioli, IM
Sfeir, Ccsfeir@pitt.eduCSFEIR
Owyang, H
Buzalaf, MAR
Vieira, ARarv11@pitt.eduARV11
ContributionContributors NameEmailPitt UsernameORCID
Date: 1 December 2015
Date Type: Publication
Access Restriction: No restriction; Release the ETD for access worldwide immediately.
Journal or Publication Title: PLoS ONE
Volume: 10
Number: 12
DOI or Unique Handle: 10.1371/journal.pone.0143068
Institution: University of Pittsburgh
Schools and Programs: School of Dental Medicine > Dental Science
Refereed: Yes
Date Deposited: 23 Aug 2016 14:53
Last Modified: 26 May 2022 11:55


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