Hammond, GRV and Takasuga, S and Sasaki, T and Balla, T
(2015)
The ML1Nx2 phosphatidylinositol 3,5-bisphosphate probe shows poor selectivity in cells.
PLoS ONE, 10 (10).
Abstract
Phosphatidylinositol (3,5)-bisphosphate (PtdIns(3,5)P2) is a quantitatively minor phospholipid in eukaryotic cells that plays a fundamental role in regulating endocytic membrane traffic. Despite its clear importance for cellular function and organism physiology, mechanistic details of its biology have so far not been fully elucidated. In part, this is due to a lack of experimental tools that specifically probe for PtdIns(3,5)P2 in cells to unambiguously identify its dynamics and site(s) of action. In this study, we have evaluated a recently reported PtdIns(3,5)P2 biosensor, GFP-ML1Nx2, for its veracity as such a probe. We report that, in live cells, the localization of this biosensor to sub-cellular compartments is largely independent of PtdIns(3,5)P2, as assessed after pharmacological, chemical genetic or genomic interventions that block the lipid's synthesis. We therefore conclude that it is unwise to interpret the localization of ML1Nx2 as a true and unbiased biosensor for PtdIns(3,5)P2.
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Item Type: |
Article
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Status: |
Published |
Creators/Authors: |
Creators | Email | Pitt Username | ORCID ![](/images/orcid_id_24x24.png) |
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Hammond, GRV | ghammond@pitt.edu | GHAMMOND | | Takasuga, S | | | | Sasaki, T | | | | Balla, T | | | |
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Contributors: |
Contribution | Contributors Name | Email | Pitt Username | ORCID ![](/images/orcid_id_24x24.png) |
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Editor | Falasca, Marco | UNSPECIFIED | UNSPECIFIED | UNSPECIFIED |
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Date: |
13 October 2015 |
Date Type: |
Publication |
Access Restriction: |
No restriction; Release the ETD for access worldwide immediately. |
Journal or Publication Title: |
PLoS ONE |
Volume: |
10 |
Number: |
10 |
DOI or Unique Handle: |
10.1371/journal.pone.0139957 |
Institution: |
University of Pittsburgh |
Schools and Programs: |
School of Medicine > Cell Biology |
Refereed: |
Yes |
Date Deposited: |
23 Aug 2016 14:42 |
Last Modified: |
22 Jun 2021 10:55 |
URI: |
http://d-scholarship.pitt.edu/id/eprint/28376 |
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