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The ML1Nx2 phosphatidylinositol 3,5-bisphosphate probe shows poor selectivity in cells

Hammond, GRV and Takasuga, S and Sasaki, T and Balla, T (2015) The ML1Nx2 phosphatidylinositol 3,5-bisphosphate probe shows poor selectivity in cells. PLoS ONE, 10 (10).

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Abstract

Phosphatidylinositol (3,5)-bisphosphate (PtdIns(3,5)P2) is a quantitatively minor phospholipid in eukaryotic cells that plays a fundamental role in regulating endocytic membrane traffic. Despite its clear importance for cellular function and organism physiology, mechanistic details of its biology have so far not been fully elucidated. In part, this is due to a lack of experimental tools that specifically probe for PtdIns(3,5)P2 in cells to unambiguously identify its dynamics and site(s) of action. In this study, we have evaluated a recently reported PtdIns(3,5)P2 biosensor, GFP-ML1Nx2, for its veracity as such a probe. We report that, in live cells, the localization of this biosensor to sub-cellular compartments is largely independent of PtdIns(3,5)P2, as assessed after pharmacological, chemical genetic or genomic interventions that block the lipid's synthesis. We therefore conclude that it is unwise to interpret the localization of ML1Nx2 as a true and unbiased biosensor for PtdIns(3,5)P2.


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Details

Item Type: Article
Status: Published
Creators/Authors:
CreatorsEmailPitt UsernameORCID
Hammond, GRVghammond@pitt.eduGHAMMOND
Takasuga, S
Sasaki, T
Balla, T
Contributors:
ContributionContributors NameEmailPitt UsernameORCID
EditorFalasca, MarcoUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Date: 13 October 2015
Date Type: Publication
Access Restriction: No restriction; Release the ETD for access worldwide immediately.
Journal or Publication Title: PLoS ONE
Volume: 10
Number: 10
DOI or Unique Handle: 10.1371/journal.pone.0139957
Institution: University of Pittsburgh
Schools and Programs: School of Medicine > Cell Biology
Refereed: Yes
Date Deposited: 23 Aug 2016 14:42
Last Modified: 02 Feb 2019 13:58
URI: http://d-scholarship.pitt.edu/id/eprint/28376

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