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Definitive characterization of CA 19-9 in resectable pancreatic cancer using a reference set of serum and plasma specimens

Haab, BB and Huang, Y and Balasenthil, S and Partyka, K and Tang, H and Anderson, M and Allen, P and Sasson, A and Zeh, H and Kaul, K and Kletter, D and Ge, S and Bern, M and Kwon, R and Blasutig, I and Srivastava, S and Frazier, ML and Sen, S and Hollingsworth, MA and Rinaudo, JA and Killary, AM and Brand, RE (2015) Definitive characterization of CA 19-9 in resectable pancreatic cancer using a reference set of serum and plasma specimens. PLoS ONE, 10 (10).

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The validation of candidate biomarkers often is hampered by the lack of a reliable means of assessing and comparing performance. We present here a reference set of serum and plasma samples to facilitate the validation of biomarkers for resectable pancreatic cancer. The reference set includes a large cohort of stage I-II pancreatic cancer patients, recruited from 5 different institutions, and relevant control groups. We characterized the performance of the current best serological biomarker for pancreatic cancer, CA 19-9, using plasma samples from the reference set to provide a benchmark for future biomarker studies and to further our knowledge of CA 19-9 in early-stage pancreatic cancer and the control groups. CA 19-9 distinguished pancreatic cancers from the healthy and chronic pancreatitis groups with an average sensitivity and specificity of 70-74%, similar to previous studies using all stages of pancreatic cancer. Chronic pancreatitis patients did not show CA 19-9 elevations, but patients with benign biliary obstruction had elevations nearly as high as the cancer patients. We gained additional information about the biomarker by comparing two distinct assays. The two CA 9-9 assays agreed well in overall performance but diverged in measurements of individual samples, potentially due to subtle differences in antibody specificity as revealed by glycan array analysis. Thus, the reference set promises be a valuable resource for biomarker validation and comparison, and the CA 19-9 data presented here will be useful for benchmarking and for exploring relationships to CA 19-9.


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Item Type: Article
Status: Published
CreatorsEmailPitt UsernameORCID
Haab, BB
Huang, Y
Balasenthil, S
Partyka, K
Tang, H
Anderson, M
Allen, P
Sasson, A
Zeh, Hhjz1@pitt.eduHJZ1
Kaul, K
Kletter, D
Ge, S
Bern, M
Kwon, R
Blasutig, I
Srivastava, S
Frazier, ML
Sen, S
Hollingsworth, MA
Rinaudo, JA
Killary, AM
Brand, REreb53@pitt.eduREB53
Date: 2 October 2015
Date Type: Publication
Access Restriction: No restriction; Release the ETD for access worldwide immediately.
Journal or Publication Title: PLoS ONE
Volume: 10
Number: 10
DOI or Unique Handle: 10.1371/journal.pone.0139049
Institution: University of Pittsburgh
Refereed: Yes
Date Deposited: 23 Aug 2016 14:39
Last Modified: 02 Feb 2019 17:55


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