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Inhaled carbon monoxide protects against the development of shock and mitochondrial injury following hemorrhage and resuscitation

Gomez, H and Kautza, B and Escobar, D and Nassour, I and Luciano, J and Botero, AM and Gordon, L and Martinez, S and Holder, A and Ogundele, O and Loughran, P and Rosengart, MR and Pinsky, M and Shiva, S and Zuckerbraun, BS (2015) Inhaled carbon monoxide protects against the development of shock and mitochondrial injury following hemorrhage and resuscitation. PLoS ONE, 10 (9).

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Abstract

Aims: Currently, there is no effective resuscitative adjunct to fluid and blood products to limit tissue injury for traumatic hemorrhagic shock. The objective of this study was to investigate the role of inhaled carbon monoxide (CO) to limit inflammation and tissue injury, and specifically mitochondrial damage, in experimental models of hemorrhage and resuscitation. Results: Inhaled CO (250 ppm for 30 minutes) protected against mortality in severe murine hemorrhagic shock and resuscitation (HS/R) (20% vs. 80%; P<0.01). Additionally, CO limited the development of shock as determined by arterial blood pH (7.25±0.06 vs. 7.05±0.05; P<0.05), lactate levels (7.2±5.1 vs 13.3±6.0; P<0.05), and base deficit (13±3.0 vs 24±3.1; P<0.05). A dose response of CO (25-500 ppm) demonstrated protection against HS/R lung and liver injury as determined by MPO activity and serum ALT, respectively. CO limited HS/R-induced increases in serum tumor necrosis factor-α and interleukin-6 levels as determined by ELISA (P<0.05 for doses of 100-500ppm). Furthermore, inhaled CO limited HS/R induced oxidative stress as determined by hepatic oxidized glutathione:reduced glutathione levels and lipid peroxidation. In porcine HS/R, CO did not influence hemodynamics. However, CO limited HS/R-induced skeletal muscle and platelet mitochondrial injury as determined by respiratory control ratio (muscle) and ATP-linked respiration and mitochondrial reserve capacity (platelets). Conclusion: These preclinical studies suggest that inhaled CO can be a protective therapy in HS/R; however, further clinical studies are warranted.

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Details

Item Type: Article
Status: Published
Creators/Authors:
CreatorsEmailPitt UsernameORCID
Gomez, H
Kautza, B
Escobar, D
Nassour, I
Luciano, J
Botero, AM
Gordon, L
Martinez, S
Holder, Aholder@pitt.eduHOLDER
Ogundele, O
Loughran, Ppal32@pitt.eduPAL320000-0001-9375-1724
Rosengart, MRmrr18@pitt.eduMRR18
Pinsky, Mpinsky@pitt.eduPINSKY0000-0001-6166-700X
Shiva, Ssss43@pitt.eduSSS43
Zuckerbraun, BS
Contributors:
ContributionContributors NameEmailPitt UsernameORCID
EditorRaju, RaghavanUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Centers: Other Centers, Institutes, Offices, or Units > Vascular Medicine Institute
Date: 14 September 2015
Date Type: Publication
Access Restriction: No restriction; Release the ETD for access worldwide immediately.
Journal or Publication Title: PLoS ONE
Volume: 10
Number: 9
DOI or Unique Handle: 10.1371/journal.pone.0135032
Institution: University of Pittsburgh
Schools and Programs: School of Medicine > Critical Care Medicine
School of Medicine > Pharmacology and Chemical Biology
School of Medicine > Surgery
Refereed: Yes
Date Deposited: 23 Aug 2016 14:38
Last Modified: 06 Oct 2021 14:55
URI: http://d-scholarship.pitt.edu/id/eprint/28386

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