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An unusual phage repressor encoded by mycobacteriophage BPs

Villanueva, VM and Oldfield, LM and Hatfull, GF (2015) An unusual phage repressor encoded by mycobacteriophage BPs. PLoS ONE, 10 (9).

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Abstract

© 2015 Villanueva et al. Temperate bacteriophages express transcription repressors that maintain lysogeny by down-regulating lytic promoters and confer superinfection immunity. Repressor regulation is critical to the outcome of infection - lysogenic or lytic growth - as well as prophage induction into lytic replication. Mycobacteriophage BPs and its relatives use an unusual integration- dependent immunity system in which the phage attachment site (attP) is located within the repressor gene (33) such that site-specific integration leads to synthesis of a prophageencoded product (gp33 103 ) that is 33 residues shorter at its C-terminus than the virallyencoded protein (gp33 136 ). However, the shorter form of the repressor (gp33 103 ) is stable and active in repression of the early lytic promoter PR, whereas the longer virally-encoded form (gp33 136 ) is inactive due to targeted degradation via a C-terminal ssrA-like tag. We show here that both forms of the repressor bind similarly to the 33-34 intergenic regulatory region, and that BPs gp33 103 is a tetramer in solution. The BPs gp33 103 repressor binds to five regulatory regions spanning the BPs genome, and regulates four promoters including the early lytic promoter, PR. BPs gp33 103 has a complex pattern of DNA recognition in which a full operator binding site contains two half sites separated by a variable spacer, and BPs gp33 103 induces a DNA bend at the full operator site but not a half site. The operator site structure is unusual in that one half site corresponds to a 12 bp palindrome identified previously, but the other half site is a highly variable variant of the palindrome. Copyright:


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Details

Item Type: Article
Status: Published
Creators/Authors:
CreatorsEmailPitt UsernameORCID
Villanueva, VMvmv6@pitt.eduVMV6
Oldfield, LM
Hatfull, GFgfh@pitt.eduGFH
Contributors:
ContributionContributors NameEmailPitt UsernameORCID
EditorSemsey, SzabolcsUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Date: 2 September 2015
Date Type: Publication
Access Restriction: No restriction; Release the ETD for access worldwide immediately.
Journal or Publication Title: PLoS ONE
Volume: 10
Number: 9
DOI or Unique Handle: 10.1371/journal.pone.0137187
Institution: University of Pittsburgh
Schools and Programs: Dietrich School of Arts and Sciences > Biological Sciences
Refereed: Yes
Date Deposited: 23 Aug 2016 14:37
Last Modified: 11 May 2019 14:55
URI: http://d-scholarship.pitt.edu/id/eprint/28391

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