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An unusual phage repressor encoded by mycobacteriophage BPs

Villanueva, VM and Oldfield, LM and Hatfull, GF (2015) An unusual phage repressor encoded by mycobacteriophage BPs. PLoS ONE, 10 (9).

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Temperate bacteriophages express transcription repressors that maintain lysogeny by down-regulating lytic promoters and confer superinfection immunity. Repressor regulation is critical to the outcome of infection - lysogenic or lytic growth - as well as prophage induction into lytic replication. Mycobacteriophage BPs and its relatives use an unusual integration- dependent immunity system in which the phage attachment site (attP) is located within the repressor gene (33) such that site-specific integration leads to synthesis of a prophageencoded product (gp33103) that is 33 residues shorter at its C-terminus than the virallyencoded protein (gp33136). However, the shorter form of the repressor (gp33103) is stable and active in repression of the early lytic promoter PR, whereas the longer virally-encoded form (gp33136) is inactive due to targeted degradation via a C-terminal ssrA-like tag. We show here that both forms of the repressor bind similarly to the 33-34 intergenic regulatory region, and that BPs gp33103 is a tetramer in solution. The BPs gp33103 repressor binds to five regulatory regions spanning the BPs genome, and regulates four promoters including the early lytic promoter, PR. BPs gp33103 has a complex pattern of DNA recognition in which a full operator binding site contains two half sites separated by a variable spacer, and BPs gp33103 induces a DNA bend at the full operator site but not a half site. The operator site structure is unusual in that one half site corresponds to a 12 bp palindrome identified previously, but the other half site is a highly variable variant of the palindrome. Copyright:


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Item Type: Article
Status: Published
CreatorsEmailPitt UsernameORCID
Villanueva, VMvmv6@pitt.eduVMV6
Oldfield, LM
Hatfull, GFgfh@pitt.eduGFH
ContributionContributors NameEmailPitt UsernameORCID
Date: 2 September 2015
Date Type: Publication
Access Restriction: No restriction; Release the ETD for access worldwide immediately.
Journal or Publication Title: PLoS ONE
Volume: 10
Number: 9
DOI or Unique Handle: 10.1371/journal.pone.0137187
Institution: University of Pittsburgh
Schools and Programs: Dietrich School of Arts and Sciences > Biological Sciences
Refereed: Yes
Date Deposited: 23 Aug 2016 14:37
Last Modified: 22 Jun 2021 12:56


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