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C/EBPβ promotes immunity to oral candidiasis through regulation of β-defensins

Simpson-Abelson, MR and Childs, EE and Ferreira, MC and Bishu, S and Conti, HR and Gaffen, SL (2015) C/EBPβ promotes immunity to oral candidiasis through regulation of β-defensins. PLoS ONE, 10 (8).

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Abstract

© 2015 Simpson-Abelson et al. Humans or mice subjected to immunosuppression, such as corticosteroids or anti-cytokine biologic therapies, are susceptible to mucosal infections by the commensal fungus Candida albicans. Recently it has become evident that the Th17/IL-17 axis is essential for immunity to candidiasis, but the downstream events that control immunity to this fungus are poorly understood. The CCAAT/Enhancer Binding Protein-β (C/EBPβ) transcription factor is important for signaling by multiple inflammatory stimuli, including IL-17. C/EBPβ is regulated in a variety of ways by IL-17, and controls several downstream IL-17 target genes. However, the role of C/EBPβ in vivo is poorly understood, in part because C/EBPβ-deficient mice are challenging to breed and work with. In this study, we sought to understand the role of C/EBPβ in the context of an IL-17-dependent immune response, using C. albicans infection as a model system. Confirming prior findings, we found that C/EBPβ is required for immunity to systemic candidiasis. In contrast, C/EBPβ-/- mice were resistant to oropharyngeal candidiasis (OPC), in a manner indistinguishable from immunocompetent WT mice. However, C/EBPβ-/- mice experienced more severe OPC than WT mice in the context of cortisoneinduced immunosuppression. Expression of the antimicrobial peptide β-defensin (BD)-3 correlated strongly with susceptibility in C/EBPβ-/- mice, but no other IL-17-dependent genes were associated with susceptibility. Therefore, C/EBPβ contributes to immunity to mucosal candidiasis during cortisone immunosuppression in a manner linked to β-defensin 3 expression, but is apparently dispensable for the IL-17-dependent response. Copyright:


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Details

Item Type: Article
Status: Published
Creators/Authors:
CreatorsEmailPitt UsernameORCID
Simpson-Abelson, MR
Childs, EEeecst@pitt.eduEECST
Ferreira, MC
Bishu, S
Conti, HR
Gaffen, SLsarah.gaffen@pitt.eduSIG65
Contributors:
ContributionContributors NameEmailPitt UsernameORCID
EditorNaglik, Julian R.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Date: 28 August 2015
Date Type: Publication
Access Restriction: No restriction; Release the ETD for access worldwide immediately.
Journal or Publication Title: PLoS ONE
Volume: 10
Number: 8
DOI or Unique Handle: 10.1371/journal.pone.0136538
Institution: University of Pittsburgh
Schools and Programs: School of Medicine > Immunology
Refereed: Yes
Date Deposited: 23 Aug 2016 14:29
Last Modified: 04 Feb 2019 15:55
URI: http://d-scholarship.pitt.edu/id/eprint/28393

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