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Platelets from asthmatic individuals show less reliance on glycolysis

Xu, W and Cardenes, N and Corey, C and Erzurum, SC and Shiva, S (2015) Platelets from asthmatic individuals show less reliance on glycolysis. PLoS ONE, 10 (7).

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Abstract

© 2015 Xu et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Asthma, a chronic inflammatory airway disease, is typified by high levels of T < inf > H < /inf > 2-cytokines and excessive generation of reactive nitrogen and oxygen species, which contribute to bronchial epithelial injury and airway remodeling. While immune function plays a major role in the pathogenesis of the disease, accumulating evidence suggests that altered cellular metabolism is a key determinant in the predisposition and disease progression of asthma. Further, several studies demonstrate altered mitochondrial function in asthmatic airways and suggest that these changes may be systemic. However, it is unknown whether systemic metabolic changes can be detected in circulating cells in asthmatic patients. Platelets are easily accessible blood cells that are known to propagate airway inflammation in asthma. Here we perform a bioenergetic screen of platelets from asthmatic and healthy individuals and demonstrate that asthmatic platelets show a decreased reliance on glycolytic processes and have increased tricarboxylic acid cycle activity. These data demonstrate a systemic alteration in asthma and are consistent with prior reports suggesting that oxidative phosphorylation is more efficient asthmatic individuals. The implications for this potential metabolic shift will be discussed in the context of increased oxidative stress and hypoxic adaptation of asthmatic patients. Further, these data suggest that platelets are potentially a good model for the monitoring of bioenergetic changes in asthma.


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Details

Item Type: Article
Status: Published
Creators/Authors:
CreatorsEmailPitt UsernameORCID
Xu, W
Cardenes, Nnac50@pitt.eduNAC50
Corey, Ccgc9@pitt.eduCGC9
Erzurum, SC
Shiva, Ssss43@pitt.eduSSS43
Contributors:
ContributionContributors NameEmailPitt UsernameORCID
EditorZhang, JianhuaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Centers: Other Centers, Institutes, or Units > Vascular Medicine Institute
Date: 6 July 2015
Date Type: Publication
Access Restriction: No restriction; Release the ETD for access worldwide immediately.
Journal or Publication Title: PLoS ONE
Volume: 10
Number: 7
DOI or Unique Handle: 10.1371/journal.pone.0132007
Institution: University of Pittsburgh
Schools and Programs: School of Medicine > Pharmacology and Chemical Biology
Refereed: Yes
Date Deposited: 23 Aug 2016 14:21
Last Modified: 16 Oct 2017 02:55
URI: http://d-scholarship.pitt.edu/id/eprint/28423

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