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Downregulation of the Host Gene jigr1 by miR-92 Is Essential for Neuroblast Self-Renewal in Drosophila

Yuva-Aydemir, Y and Xu, XL and Aydemir, O and Gascon, E and Sayin, S and Zhou, W and Hong, Y and Gao, FB (2015) Downregulation of the Host Gene jigr1 by miR-92 Is Essential for Neuroblast Self-Renewal in Drosophila. PLoS Genetics, 11 (5). ISSN 1553-7390

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Abstract

Intragenic microRNAs (miRNAs), located mostly in the introns of protein-coding genes, are often co-expressed with their host mRNAs. However, their functional interaction in development is largely unknown. Here we show that in Drosophila, miR-92a and miR-92b are embedded in the intron and 3’UTR of jigr1, respectively, and co-expressed with some jigr1 isoforms. miR-92a and miR-92b are highly expressed in neuroblasts of larval brain where Jigr1 expression is low. Genetic deletion of both miR-92a and miR-92b demonstrates an essential cell-autonomous role for these miRNAs in maintaining neuroblast self-renewal through inhibiting premature differentiation. We also show that miR-92a and miR-92b directly target jigr1 in vivo and that some phenotypes due to the absence of these miRNAs are partially rescued by reducing the level of jigr1. These results reveal a novel function of the miR-92 family in Drosophila neuroblasts and provide another example that local negative feedback regulation of host genes by intragenic miRNAs is essential for animal development.

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Details

Item Type: Article
Status: Published
Creators/Authors:
CreatorsEmailPitt UsernameORCID
Yuva-Aydemir, Y
Xu, XL
Aydemir, O
Gascon, E
Sayin, S
Zhou, W
Hong, Yyhong@pitt.eduYHONG
Gao, FB
Contributors:
ContributionContributors NameEmailPitt UsernameORCID
EditorLu, BingweiUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Date: 1 May 2015
Date Type: Publication
Access Restriction: No restriction; Release the ETD for access worldwide immediately.
Journal or Publication Title: PLoS Genetics
Volume: 11
Number: 5
DOI or Unique Handle: 10.1371/journal.pgen.1005264
Institution: University of Pittsburgh
Schools and Programs: School of Medicine > Cell Biology
Refereed: Yes
ISSN: 1553-7390
Date Deposited: 05 Jul 2016 20:37
Last Modified: 23 Nov 2021 16:55
URI: http://d-scholarship.pitt.edu/id/eprint/28524

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