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Characterization of mycobacteria and mycobacteriophages isolated from compost at the São Paulo Zoo Park Foundation in Brazil and creation of the new mycobacteriophage Cluster U

Lima-Junior, JD and Viana-Niero, C and Conde Oliveira, DV and Machado, GE and Rabello, MCDS and Martins-Junior, J and Martins, LF and Digiampietri, LA and Da Silva, AM and Setubal, JC and Russell, DA and Jacobs-Sera, D and Pope, WH and Hatfull, GF and Leão, SC (2016) Characterization of mycobacteria and mycobacteriophages isolated from compost at the São Paulo Zoo Park Foundation in Brazil and creation of the new mycobacteriophage Cluster U. BMC Microbiology, 16 (1).

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Abstract

© 2016 The Author(s). Background: A large collection of sequenced mycobacteriophages capable of infecting a single host strain of Mycobacterium smegmatis shows considerable genomic diversity with dozens of distinctive types (clusters) and extensive variation within those sharing evident nucleotide sequence similarity. Here we profiled the mycobacterial components of a large composting system at the São Paulo zoo. Results: We isolated and sequenced eight mycobacteriophages using Mycobacterium smegmatis mc2155 as a host. None of these eight phages infected any of mycobacterial strains isolated from the same materials. The phage isolates span considerable genomic diversity, including two phages (Barriga, Nhonho) related to Subcluster A1 phages, two Cluster B phages (Pops, Subcluster B1; Godines, Subcluster B2), three Subcluster F1 phages (Florinda, Girafales, and Quico), and Madruga, a relative of phage Patience with which it constitutes the new Cluster U. Interestingly, the two Subcluster A1 phages and the three Subcluster F1 phages have genomic relationships indicating relatively recent evolution within a geographically isolated niche in the composting system. Conclusions: We predict that composting systems such as those used to obtain these mycobacteriophages will be a rich source for the isolation of additional phages that will expand our view of bacteriophage diversity and evolution.


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Details

Item Type: Article
Status: Published
Creators/Authors:
CreatorsEmailPitt UsernameORCID
Lima-Junior, JD
Viana-Niero, C
Conde Oliveira, DV
Machado, GE
Rabello, MCDS
Martins-Junior, J
Martins, LF
Digiampietri, LA
Da Silva, AM
Setubal, JC
Russell, DAdar78@pitt.eduDAR780000-0001-6894-2748
Jacobs-Sera, Ddjs@pitt.eduDJS0000-0001-7838-3257
Pope, WHwelkin@pitt.eduWELKIN0000-0002-2012-2924
Hatfull, GFgfh@pitt.eduGFH
Leão, SC
Date: 17 June 2016
Date Type: Publication
Access Restriction: No restriction; Release the ETD for access worldwide immediately.
Journal or Publication Title: BMC Microbiology
Volume: 16
Number: 1
DOI or Unique Handle: 10.1186/s12866-016-0734-3
Institution: University of Pittsburgh
Schools and Programs: Dietrich School of Arts and Sciences > Biological Sciences
Refereed: Yes
Date Deposited: 25 Jul 2016 13:51
Last Modified: 02 Feb 2019 15:57
URI: http://d-scholarship.pitt.edu/id/eprint/28623

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