Link to the University of Pittsburgh Homepage
Link to the University Library System Homepage Link to the Contact Us Form

Regulation of chaperone binding and nucleosome dynamics by key residues within the globular domain of histone H3

Hainer, SJ and Martens, JA (2016) Regulation of chaperone binding and nucleosome dynamics by key residues within the globular domain of histone H3. Epigenetics and Chromatin, 9 (1).

[img]
Preview
PDF
Published Version
Available under License : See the attached license file.

Download (2MB)
[img] Plain Text (licence)
Available under License : See the attached license file.

Download (1kB)

Abstract

© 2016 Hainer and Martens. Background: Nucleosomes have an important role in modulating access of DNA by regulatory factors. The role specific histone residues have in this process has been shown to be an important mechanism of transcription regulation. Previously, we identified eight amino acids in histones H3 and H4 that are required for nucleosome occupancy over highly transcribed regions of the genome. Results: We investigate the mechanism through which three of these previously identified histone H3 amino acids regulate nucleosome architecture. We find that histone H3 K122, Q120, and R49 are required for Spt2, Spt6, and Spt16 occupancies at genomic locations where transcription rates are high, but not over regions of low transcription rates. Furthermore, substitution at one residue, K122, located on the dyad axis of the nucleosome, results in improper reassembly and disassembly of nucleosomes, likely accounting for the transcription rate-dependent regulation by these mutant histones. Conclusions: These data show that when specific amino acids of histone proteins are substituted, Spt2, Spt6, and Spt16 occupancies are reduced and nucleosome dynamics are altered. Therefore, these data support a mechanism for histone chaperone binding where these factors interact with histone proteins to promote their activities during transcription.


Share

Citation/Export:
Social Networking:
Share |

Details

Item Type: Article
Status: Published
Creators/Authors:
CreatorsEmailPitt UsernameORCID
Hainer, SJsarah.hainer@pitt.eduSHAINER0000-0003-0503-1183
Martens, JA
Date: 1 January 2016
Date Type: Publication
Access Restriction: No restriction; Release the ETD for access worldwide immediately.
Journal or Publication Title: Epigenetics and Chromatin
Volume: 9
Number: 1
DOI or Unique Handle: 10.1186/s13072-016-0066-4
Institution: University of Pittsburgh
Schools and Programs: Dietrich School of Arts and Sciences > Biological Sciences
Dietrich School of Arts and Sciences > Molecular Biology
School of Medicine > Cell Biology
Refereed: Yes
Date Deposited: 21 Jul 2016 20:39
Last Modified: 29 Jan 2019 15:55
URI: http://d-scholarship.pitt.edu/id/eprint/28674

Metrics

Monthly Views for the past 3 years

Plum Analytics

Altmetric.com


Actions (login required)

View Item View Item