Link to the University of Pittsburgh Homepage
Link to the University Library System Homepage Link to the Contact Us Form

On Predicting lung cancer subtypes using 'omic' data from tumor and tumor-adjacent histologically-normal tissue

Pineda, AL and Ogoe, HA and Balasubramanian, JB and Rangel Escareño, C and Visweswaran, S and Herman, JG and Gopalakrishnan, V (2016) On Predicting lung cancer subtypes using 'omic' data from tumor and tumor-adjacent histologically-normal tissue. BMC Cancer, 16 (1).

Published Version
Available under License : See the attached license file.

Download (1MB) | Preview
[img] Plain Text (licence)
Available under License : See the attached license file.

Download (1kB)


Background: Adenocarcinoma (ADC) and squamous cell carcinoma (SCC) are the most prevalent histological types among lung cancers. Distinguishing between these subtypes is critically important because they have different implications for prognosis and treatment. Normally, histopathological analyses are used to distinguish between the two, where the tissue samples are collected based on small endoscopic samples or needle aspirations. However, the lack of cell architecture in these small tissue samples hampers the process of distinguishing between the two subtypes. Molecular profiling can also be used to discriminate between the two lung cancer subtypes, on condition that the biopsy is composed of at least 50% of tumor cells. However, for some cases, the tissue composition of a biopsy might be a mix of tumor and tumor-adjacent histologically normal tissue (TAHN). When this happens, a new biopsy is required, with associated cost, risks and discomfort to the patient. To avoid this problem, we hypothesize that a computational method can distinguish between lung cancer subtypes given tumor and TAHN tissue. Methods: Using publicly available datasets for gene expression and DNA methylation, we applied four classification tasks, depending on the possible combinations of tumor and TAHN tissue. First, we used a feature selector (ReliefF/Limma) to select relevant variables, which were then used to build a simple naïve Bayes classification model. Then, we evaluated the classification performance of our models by measuring the area under the receiver operating characteristic curve (AUC). Finally, we analyzed the relevance of the selected genes using hierarchical clustering and IPA® software for gene functional analysis. Results: All Bayesian models achieved high classification performance (AUC>0.94), which were confirmed by hierarchical cluster analysis. From the genes selected, 25 (93%) were found to be related to cancer (19 were associated with ADC or SCC), confirming the biological relevance of our method. Conclusions: The results from this study confirm that computational methods using tumor and TAHN tissue can serve as a prognostic tool for lung cancer subtype classification. Our study complements results from other studies where TAHN tissue has been used as prognostic tool for prostate cancer. The clinical implications of this finding could greatly benefit lung cancer patients.


Social Networking:
Share |


Item Type: Article
Status: Published
CreatorsEmailPitt UsernameORCID
Pineda, AL
Ogoe, HAhao9@pitt.eduHAO9
Balasubramanian, JBjeya@pitt.eduJEYA
Rangel Escareño, C
Visweswaran, Sshv3@pitt.eduSHV3
Herman, JG
Gopalakrishnan, Vvanathi@pitt.eduVANATHI
Date: 4 March 2016
Date Type: Publication
Journal or Publication Title: BMC Cancer
Volume: 16
Number: 1
DOI or Unique Handle: 10.1186/s12885-016-2223-3
Schools and Programs: School of Medicine > Biomedical Informatics
Refereed: Yes
Date Deposited: 22 Aug 2016 19:09
Last Modified: 30 Mar 2021 13:56


Monthly Views for the past 3 years

Plum Analytics

Actions (login required)

View Item View Item