Lipid levels in HIV-positive men receiving anti-retroviral therapy are not associated with copy number variation of reverse cholesterol transport pathway genes Genetics

Marino, RB and Kingsley, LA and Hussain, SK and Bream, JH and Penogonda, S and Duggal, P and Martinson, JJ (2015) Lipid levels in HIV-positive men receiving anti-retroviral therapy are not associated with copy number variation of reverse cholesterol transport pathway genes Genetics. BMC Research Notes, 8 (1).

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Abstract

Background: The exacerbation of HIV-1 associated dyslipidemia seen in a subset of patients receiving anti-retroviral therapy suggests that genetic factors put these individuals at greater risk of cardiovascular disease. Single nucleotide polymorphisms (SNPs) within genes of and influencing the reverse cholesterol transport (RCT) pathway are associated with lipid levels but little is known regarding their copy number variation (CNV). This form of quantitative genetic variation has the potential to alter the amount of gene product made, thereby also influencing lipid metabolism. Results: To examine if CNV in RCT pathway genes was associated with altered serum lipid profiles in HIV-positive individuals receiving therapy, we designed a custom multiplex ligation-dependent probe amplification assay to screen 16 RCT genes within a subset of individuals from the Multicenter AIDS Cohort Study who show extreme lipid phenotypes. Verification of CNV was performed using a custom NanoString assay, and the Illumina HT-12 mRNA expression microarray was used to determine the influence of copy number on gene expression. Among the RCT genes, CNV was observed to be extremely rare. The only CNV seen was in the CETP gene, which showed a loss of copy in 1 of the 320 samples (0.3 %) in our study. The genes in our study showed little variation in expression between individuals, and the variation seen was not related to any detected CNV. Conclusions: Whole gene CNV is uncommon in RCT pathway genes, and not a major factor in the development of highly active antiretroviral therapy (HAART) associated dyslipidemia.

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Details

Item Type:	Article
Status:	Published
Creators/Authors:	Creators Email Pitt Username ORCID Marino, RB rsb24@pitt.edu RSB24 Kingsley, LA kingsley@pitt.edu KINGSLEY Hussain, SK Bream, JH Penogonda, S Duggal, P Martinson, JJ jmartins@pitt.edu JMARTINS
Date:	21 November 2015
Date Type:	Publication
Journal or Publication Title:	BMC Research Notes
Volume:	8
Number:	1
DOI or Unique Handle:	10.1186/s13104-015-1665-z
Schools and Programs:	School of Medicine > Infectious Diseases and Microbiology
Refereed:	Yes
Date Deposited:	26 Jul 2016 20:04
Last Modified:	30 Mar 2021 12:55
URI:	http://d-scholarship.pitt.edu/id/eprint/28952

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