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A MINIMAL CONSERVED REGION OF SHROOM2 AND SHROOM3 MEDIATES ACTIN BINDING AND BUNDLING

Cleveland-Rubeor, Hillary C. (2016) A MINIMAL CONSERVED REGION OF SHROOM2 AND SHROOM3 MEDIATES ACTIN BINDING AND BUNDLING. Master's Thesis, University of Pittsburgh. (Unpublished)

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Abstract

Changes in cell morphology are essential for generating the complex tissues that make up all organisms. Regulation of the cell’s morphology is dynamic and is achieved through the coordinated activity of numerous signaling pathways and proteins that converge on the cytoskeleton at many levels. In epithelial cells, the Shroom family of proteins drives anisotropic changes to morphology such as apical constriction and convergent extension in the formation of such structures as the neural tube, vasculature, gut, and eye. Shroom proteins are a class of actin-binding proteins that recruit Rho-associated coiled-coil Kinase (or Rock), to the cytoskeleton to induce contraction of the actin network and effect change. Recent work in this field has focused on understanding the direct interaction of Shroom and Rock, but little work has been done to understand how Shroom proteins directly interact with actin. The actin-binding ability of these proteins has been localized to Shroom Domain 1, a domain unique to Shroom proteins. My work has centered on better understanding the interaction between the SD1 and actin.
I have identified small, conserved regions of both Shroom2 and Shroom3 that are capable of binding and bundling actin with similar affinity. With the use of Transmission Electron Microscopy, I observed that both proteins organize actin filaments into similar tightly packed parallel or anti-parallel bundles. While Shroom proteins localize to different populations of actin within the cell, it appears that the ability to bind and bundle actin has been conserved, and that other region of the proteins must drive their unique localization patterns.


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Details

Item Type: University of Pittsburgh ETD
Status: Unpublished
Creators/Authors:
CreatorsEmailPitt UsernameORCID
Cleveland-Rubeor, Hillary C.hcc9@pitt.eduHCC9
ETD Committee:
TitleMemberEmail AddressPitt UsernameORCID
Committee ChairHildebrand, Jefferyjeffh@pitt.eduJEFFH
Committee MemberSchwacha, Anthonyschwacha@pitt.eduSCHWACHA
Committee MemberBoyle, Jonboylej@pitt.eduBOYLEJ
Committee MemberBerman, AndreaAJB190@pitt.eduAJB190
Date: 20 September 2016
Date Type: Publication
Defense Date: 26 May 2016
Approval Date: 20 September 2016
Submission Date: 8 August 2016
Access Restriction: 3 year -- Restrict access to University of Pittsburgh for a period of 3 years.
Number of Pages: 79
Institution: University of Pittsburgh
Schools and Programs: Dietrich School of Arts and Sciences > Biological Sciences
Degree: MS - Master of Science
Thesis Type: Master's Thesis
Refereed: Yes
Uncontrolled Keywords: Shroom, Shroom2, Shroom3, Shroom Domain 1, SD1, Actin, Actin-binding proteins, Actin-bundling proteins, TEM, Transmission Electron Microscopy
Date Deposited: 20 Sep 2016 16:04
Last Modified: 15 Nov 2016 14:35
URI: http://d-scholarship.pitt.edu/id/eprint/29174

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