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MicroRNA-940 suppresses prostate cancer migration and invasion by regulating MIEN1.

Rajendiran, Smrithi and Parwani, Anil V and Hare, Richard J and Dasgupta, Subhamoy and Roby, Rhonda K and Vishwanatha, Jamboor K (2014) MicroRNA-940 suppresses prostate cancer migration and invasion by regulating MIEN1. Mol Cancer, 13. 250 - ?.

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BACKGROUND: MicroRNAs (miRNAs) are crucial molecules that regulate gene expression and hence pathways that are key to prostate cancer progression. These non-coding RNAs are highly deregulated in prostate cancer thus facilitating progression of the disease. Among the many genes that have gained importance in this disease, Migration and invasion enhancer 1 (MIEN1), a novel gene located next to HER2/neu in the 17q12 amplicon of the human chromosome, has been shown to enhance prostate cancer cell migration and invasion, two key processes in cancer progression. MIEN1 is differentially expressed between normal and cancer cells and tissues. Understanding the regulation of MIEN1 by microRNA may enable development of better targeting strategies. METHODS: The miRNAs that could target MIEN1 were predicted by in silico algorithms and microarray analysis. The validation for miRNA expression was performed by qPCR and northern blotting in cells and by in situ hybridization in tissues. MIEN1 and levels of other molecules upon miRNA regulation was determined by Western blotting, qPCR, and immunofluorescence. The functional effects of miRNA on cells were determined by wound healing cell migration, Boyden chamber cell invasion, clonal and colony formation assays. For knockdown or overexpression of the miRNA or overexpression of MIEN1 3'UTR, cells were transfected with the oligomiRs and plasmids, respectively. RESULTS: A novel miRNA, hsa-miR-940 (miR-940), identified and validated to be highly expressed in immortalized normal cells compared to cancer cells, is a regulator of MIEN1. Analysis of human prostate tumors and their matched normal tissues confirmed that miR-940 is highly expressed in the normal tissues compared to its low to negligible expression in the tumors. While MIEN1 is a direct target of miR-940, miR-940 alters MIEN1 RNA, in a quantity as well as cell dependent context, along with altering its downstream effectors. The miR-940 inhibited migratory and invasive potential of cells, attenuated their anchorage-independent growth ability, and increased E-cadherin expression, implicating its role in mesenchymal-to-epithelial transition (MET). CONCLUSIONS: These results, for the first time, implicate miR-940, a regulator of MIEN1, as a promising novel diagnostic and prognostic tool for prostate cancer.


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Item Type: Article
Status: Published
CreatorsEmailPitt UsernameORCID
Rajendiran, Smrithi
Parwani, Anil V
Hare, Richard J
Dasgupta, Subhamoy
Roby, Rhonda K
Vishwanatha, Jamboor K
Date: 23 October 2014
Date Type: Acceptance
Journal or Publication Title: Mol Cancer
Volume: 13
Page Range: 250 - ?
DOI or Unique Handle: 10.1186/1476-4598-13-250
Schools and Programs: School of Medicine > Pathology
Refereed: Yes
Uncontrolled Keywords: Cadherins, Cell Line, Tumor, Cell Movement, Epithelial-Mesenchymal Transition, Humans, Intracellular Signaling Peptides and Proteins, Male, MicroRNAs, Neoplasm Invasiveness, Neoplasm Proteins, Prostatic Neoplasms
Funders: NIMHD NIH HHS (P20 MD006882), NIMHD NIH HHS (1P20 MD006882)
Date Deposited: 22 Dec 2016 14:52
Last Modified: 13 Oct 2017 20:56


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