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Copper chelation selectively kills colon cancer cells through redox cycling and generation of reactive oxygen species

Fatfat, M and Merhi, RA and Rahal, O and Stoyanovsky, DA and Zaki, A and Haidar, H and Kagan, VE and Gali-Muhtasib, H and Machaca, K (2014) Copper chelation selectively kills colon cancer cells through redox cycling and generation of reactive oxygen species. BMC Cancer, 14 (1).

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Abstract

Background: Metals including iron, copper and zinc are essential for physiological processes yet can be toxic at high concentrations. However the role of these metals in the progression of cancer is not well defined. Here we study the anti-tumor activity of the metal chelator, TPEN, and define its mechanism of action.Methods: Multiple approaches were employed, including cell viability, cell cycle analysis, multiple measurements of apoptosis, and mitochondrial function. In addition we measured cellular metal contents and employed EPR to record redox cycling of TPEN-metal complexes. Mouse xenografts were also performed to test the efficacy of TPEN in vivo.Results: We show that metal chelation using TPEN (5μM) selectively induces cell death in HCT116 colon cancer cells without affecting the viability of non-cancerous colon or intestinal cells. Cell death was associated with increased levels of reactive oxygen species (ROS) and was inhibited by antioxidants and by prior chelation of copper. Interestingly, HCT116 cells accumulate copper to 7-folds higher levels than normal colon cells, and the TPEN-copper complex engages in redox cycling to generate hydroxyl radicals. Consistently, TPEN exhibits robust anti-tumor activity in vivo in colon cancer mouse xenografts.Conclusion: Our data show that TPEN induces cell death by chelating copper to produce TPEN-copper complexes that engage in redox cycling to selectively eliminate colon cancer cells. © 2014 Fatfat et al.; licensee BioMed Central Ltd.


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Details

Item Type: Article
Status: Published
Creators/Authors:
CreatorsEmailPitt UsernameORCID
Fatfat, M
Merhi, RA
Rahal, O
Stoyanovsky, DAdas11@pitt.eduDAS110000-0001-5591-4780
Zaki, A
Haidar, H
Kagan, VEkagan@pitt.eduKAGAN
Gali-Muhtasib, H
Machaca, K
Date: 21 July 2014
Date Type: Publication
Journal or Publication Title: BMC Cancer
Volume: 14
Number: 1
DOI or Unique Handle: 10.1186/1471-2407-14-527
Schools and Programs: Graduate School of Public Health > Environmental and Occupational Health
Refereed: Yes
Date Deposited: 19 Sep 2016 15:44
Last Modified: 02 Feb 2019 14:57
URI: http://d-scholarship.pitt.edu/id/eprint/29509

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