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Investigation of nanoscale structural alterations of cell nucleus as an early sign of cancer

Liu, Y and Uttam, S and Alexandrov, S and Bista, RK (2014) Investigation of nanoscale structural alterations of cell nucleus as an early sign of cancer. BMC Biophysics, 7 (1). ISSN 2046-1682

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Background: The cell and tissue structural properties assessed with a conventional bright-field light microscope play a key role in cancer diagnosis, but they sometimes have limited accuracy in detecting early-stage cancers or predicting future risk of cancer progression for individual patients (i.e., prognosis) if no frank cancer is found. The recent development in optical microscopy techniques now permit the nanoscale structural imaging and quantitative structural analysis of tissue and cells, which offers a new opportunity to investigate the structural properties of cell and tissue below 200-250 nm as an early sign of carcinogenesis, prior to the presence of microscale morphological abnormalities. Identification of nanoscale structural signatures is significant for earlier and more accurate cancer detection and prognosis. Results: Our group has recently developed two simple spectral-domain optical microscopy techniques for assessing 3D nanoscale structural alterations - spectral-encoding of spatial frequency microscopy and spatial-domain low-coherence quantitative phase microscopy. These two techniques use the scattered light from biological cells and tissue and share a common experimental approach of assessing the Fourier space by various wavelengths to quantify the 3D structural information of the scattering object at the nanoscale sensitivity with a simple reflectance-mode light microscopy setup without the need for high-NA optics. This review paper discusses the physical principles and validation of these two techniques to interrogate nanoscale structural properties, as well as the use of these methods to probe nanoscale nuclear architectural alterations during carcinogenesis in cancer cell lines and well-annotated human tissue during carcinogenesis. Conclusions: The analysis of nanoscale structural characteristics has shown promise in detecting cancer before the microscopically visible changes become evident and proof-of-concept studies have shown its feasibility as an earlier or more sensitive marker for cancer detection or diagnosis. Further biophysical investigation of specific 3D nanoscale structural characteristics in carcinogenesis, especially with well-annotated human cells and tissue, is much needed in cancer research.


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Item Type: Article
Status: Published
CreatorsEmailPitt UsernameORCID
Liu, Yliuy@pitt.eduLIUY0000-0002-6388-9674
Uttam, Sshf28@pitt.eduSHF28
Alexandrov, S
Bista, RK
Centers: Other Centers, Institutes, Offices, or Units > Hillman Cancer Center
Date: 10 February 2014
Date Type: Publication
Journal or Publication Title: BMC Biophysics
Volume: 7
Number: 1
DOI or Unique Handle: 10.1186/2046-1682-7-1
Schools and Programs: School of Medicine > Medicine
Swanson School of Engineering > Bioengineering
Refereed: Yes
ISSN: 2046-1682
Date Deposited: 02 Dec 2016 20:33
Last Modified: 15 Mar 2023 11:55


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