Link to the University of Pittsburgh Homepage
Link to the University Library System Homepage Link to the Contact Us Form

Homeostatic regulation of spontaneous and evoked synaptic transmission in two steps

Gerkin, RC and Nauen, DW and Xu, F and Bi, GQ (2013) Homeostatic regulation of spontaneous and evoked synaptic transmission in two steps. Molecular Brain, 6 (1).

[img]
Preview
PDF
Published Version
Available under License : See the attached license file.

Download (634kB) | Preview
[img] Plain Text (licence)
Available under License : See the attached license file.

Download (1kB)

Abstract

Background: During development both Hebbian and homeostatic mechanisms regulate synaptic efficacy, usually working in opposite directions in response to neuronal activity. Homeostatic plasticity has often been investigated by assaying changes in spontaneous synaptic transmission resulting from chronic circuit inactivation. However, effects of inactivation on evoked transmission have been less frequently reported. Importantly, contributions from the effects of circuit inactivation and reactivation on synaptic efficacy have not been individuated. Results: Here we show for developing hippocampal neurons in primary culture that chronic inactivation with TTX results in increased mean amplitude of miniature synaptic currents (mEPSCs), but not evoked synaptic currents (eEPSCs). However, changes in quantal properties of transmission, partially reflected in mEPSCs, accurately predicted higher-order statistical properties of eEPSCs. The classical prediction of homeostasis - increased strength of evoked transmission - was realized after explicit circuit reactivation, in the form of cells' pairwise connection probability. In contrast, distributions of eEPSC amplitudes for control and inactivated-then- reactivated groups matched throughout. Conclusions: Homeostatic up-regulation of evoked synaptic transmission in developing hippocampal neurons in primary culture requires both the inactivation and reactivation stages, leading to a net increase in functional circuit connectivity. © 2013 Gerkin et al.


Share

Citation/Export:
Social Networking:
Share |

Details

Item Type: Article
Status: Published
Creators/Authors:
CreatorsEmailPitt UsernameORCID
Gerkin, RC
Nauen, DW
Xu, F
Bi, GQ
Date: 26 August 2013
Date Type: Publication
Journal or Publication Title: Molecular Brain
Volume: 6
Number: 1
DOI or Unique Handle: 10.1186/1756-6606-6-38
Schools and Programs: School of Medicine > Neurobiology
Refereed: Yes
Date Deposited: 02 Dec 2016 14:59
Last Modified: 20 Dec 2018 00:55
URI: http://d-scholarship.pitt.edu/id/eprint/29691

Metrics

Monthly Views for the past 3 years

Plum Analytics

Altmetric.com


Actions (login required)

View Item View Item