Inoue-Choi, M and Nelson, HH and Robien, K and Arning, E and Bottiglieri, T and Koh, WP and Yuan, JM
(2013)
Plasma S-adenosylmethionine, DNMT polymorphisms, and peripheral blood LINE-1 methylation among healthy Chinese adults in Singapore.
BMC Cancer, 13.
Abstract
Background: Global hypomethylation of repetitive DNA sequences is believed to occur early in tumorigenesis. There is a great interest in identifying factors that contribute to global DNA hypomethylation and associated cancer risk. We tested the hypothesis that plasma S-adenosylmethionine (SAM) level alone or in combination with genetic variation in DNA methyltransferases (DNMT1, DNMT3A and DNMT3B) was associated with global DNA methylation extent at long interspersed nucleotide element-1 (LINE-1) sequences.Methods: Plasma SAM level and LINE-1 DNA methylation index were measured using stored blood samples collected from 440 healthy Singaporean Chinese adults during 1994-1999. Genetic polymorphisms of 13 loci in DNMT1, DNMT3A and DNMT3B were determined.Results: LINE-1 methylation index was significantly higher in men than in women (p = 0.001). LINE-1 methylation index was positively associated with plasma SAM levels (p ≤ 0.01), with a plateau at approximately 78% of LINE-1 methylation index (55 nmol/L plasma SAM) in men and 77% methylation index (50 nmol/L plasma SAM) in women. In men only, the T allele of DNMT1 rs21124724 was associated with a statistically significantly higher LINE-1 methylation index (ptrend = 0.001). The DNMT1 rs2114724 genotype modified the association between plasma SAM and LINE-1 methylation index at low levels of plasma SAM in men.Conclusions: Circulating SAM level was associated with LINE-1 methylation status among healthy Chinese adults. The DNMT1 genetic polymorphism may exert a modifying effect on the association between SAM and LINE-1 methylation status in men, especially when plasma SAM level is low. Our findings support a link between plasma SAM and global DNA methylation status at LINE-1 sequences. © 2013 Inoue-Choi et al.; licensee BioMed Central Ltd.
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| Item Type: |
Article
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| Status: |
Published |
| Creators/Authors: |
| Creators | Email | Pitt Username | ORCID  |
|---|
| Inoue-Choi, M | | | | | Nelson, HH | | | | | Robien, K | | | | | Arning, E | | | | | Bottiglieri, T | | | | | Koh, WP | | | | | Yuan, JM | yuanj@pitt.edu | YUANJ | |
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| Centers: |
Other Centers, Institutes, Offices, or Units > Pittsburgh Cancer Institute |
| Date: |
17 August 2013 |
| Date Type: |
Publication |
| Journal or Publication Title: |
BMC Cancer |
| Volume: |
13 |
| DOI or Unique Handle: |
10.1186/1471-2407-13-389 |
| Schools and Programs: |
School of Public Health > Epidemiology |
| Refereed: |
Yes |
| Date Deposited: |
02 Dec 2016 14:58 |
| Last Modified: |
23 Jun 2021 09:55 |
| URI: |
http://d-scholarship.pitt.edu/id/eprint/29693 |
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