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Depletion of neutrophils in a protective model of pulmonary cryptococcosis results in increased IL-17A production by gamma/delta T cells

Wozniak, KL and Kolls, JK and Wormley, FL (2012) Depletion of neutrophils in a protective model of pulmonary cryptococcosis results in increased IL-17A production by gamma/delta T cells. BMC Immunology, 13.

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Abstract

Protective responses in mice immunized with an interferon-gamma producing strain of Cryptococcus neoformans, H99γ, are associated with IL-17A production by neutrophils. Neutrophil depletion in H99γ-immunized mice did not affect pulmonary fungal burden, indicating that neutrophils are not required for clearance. However, we observed an increase in IL-17A in the lungs of neutrophil-depleted H99γ infected mice, which corresponded to an increase in IL-17A+ γδ+ T cells. Moreover, we observed increased IL-17A+/ CD3+ cells and IL-17A+/γδ+ cells, but decreased IL-17A+/Ly6G+ neutrophils in the lungs of IL-17 receptor (R)A deficient mice compared to wild-type mice. Increased production of IL-17A in neutropenic mice coincided with increased IL-6 and CXCL1, but not Th17 inducing cytokines TGF-β, IL-21 and IL-23. Concurrent depletion of neutrophils and γδ+ T cells reduced IL-17A levels. Our results suggest that γδ+ T cells mediate significant IL-17A production in neutropenic mice during the protective response to C. neoformans infection. © 2012 Wozniak et al.; licensee BioMed Central Ltd.


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Details

Item Type: Article
Status: Published
Creators/Authors:
CreatorsEmailPitt UsernameORCID
Wozniak, KL
Kolls, JKjkk23@pitt.eduJKK23
Wormley, FL
Date: 7 December 2012
Date Type: Publication
Journal or Publication Title: BMC Immunology
Volume: 13
DOI or Unique Handle: 10.1186/1471-2172-13-65
Schools and Programs: School of Medicine > Pediatrics
Refereed: Yes
Date Deposited: 30 Nov 2016 18:39
Last Modified: 12 Feb 2019 15:55
URI: http://d-scholarship.pitt.edu/id/eprint/29792

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