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Isoflurane does not cause neuroapoptosis but reduces astroglial processes in young adult mice

Dallasen, RM and Bowman, JD and Xu, Y (2011) Isoflurane does not cause neuroapoptosis but reduces astroglial processes in young adult mice. Medical Gas Research, 1 (1).

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Background: Isoflurane, a volatile anesthetic widely used clinically, has been implicated to be both neuroprotective and neurotoxic. The claim about isoflurane causing neural apoptosis remains controversial. In this study, we investigated the effects of isoflurane exposures on apoptotic and anti-apoptotic signals, cell proliferation and neurogenesis, and astroglial processes in young adult mouse brains. Methods. Sixty 6-week-old mice were randomly assigned to four anesthetic concentration groups (0 as control and 0.6%, 1.3%, and 2%) with four recovery times (2 h and 1, 6, and 14 d) after 2-h isoflurane exposure. Immunohistochemistry measurements of activated caspase-3 and Bcl-xl for apoptotic and anti-apoptotic signals, respectively, glial fibrillary acidic protein (GFAP) and vimentin for reactive astrocytosis, doublecortin (Dcx) for neurogenesis, and BrdU for cell proliferation were performed. Results: Contrary to the previous conclusion derived from studies with neonatal rodents, we found no evidence of isoflurane-induced apoptosis in the adult mouse brain. Neurogenesis in the subgranule zone of the dentate gyrus was not affected by isoflurane. However, there is a tendency of reduced cell proliferation after 2% isoflurane exposure. VIM and GFAP staining showed that isoflurane exposure caused a delayed reduction of astroglial processes in the hippocampus and dentate gyrus. Conclusion: Two-hour exposure to isoflurane did not cause neuroapoptosis in adult brains. The delayed reduction in astroglial processes after isoflurane exposure may explain why some volatile anesthetics can confer neuroprotection after experimental stroke because reduced glial scarring facilitates better long-term neuronal recoveries. © 2011 Dallasen et al; licensee BioMed Central Ltd.


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Item Type: Article
Status: Published
CreatorsEmailPitt UsernameORCID
Dallasen, RM
Bowman, JD
Xu, Yxu2@pitt.eduXU2
Date: 1 January 2011
Date Type: Publication
Journal or Publication Title: Medical Gas Research
Volume: 1
Number: 1
DOI or Unique Handle: 10.1186/2045-9912-1-27
Schools and Programs: School of Medicine > Anesthesiology
School of Medicine > Pharmacology and Chemical Biology
School of Medicine > Structural Biology
Refereed: Yes
Date Deposited: 26 Oct 2016 18:03
Last Modified: 29 Jan 2019 15:56


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