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EGFR-specific T cell frequencies correlate with EGFR expression in head and neck squamous cell carcinoma.

Schuler, PJ and Boeckers, P and Engers, R and Boelke, E and Bas, M and Greve, J and Dumitru, CA and Lehnerdt, GF and Ferris, RL and Andrade Filho, PA and Brandau, S and Lang, S and Whiteside, TL and Hoffmann, TK (2011) EGFR-specific T cell frequencies correlate with EGFR expression in head and neck squamous cell carcinoma. Journal of translational medicine, 9. 168 - ?.

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Abstract

In head and neck squamous cell carcinoma (HNSCC), expression levels of the epidermal growth factor receptor (EGFR) correlate with poor prognosis and decreased survival rates. As the mechanisms responsible for cellular immune response to EGFR in vivo remain unclear, the frequency and function of EGFR-specific cytotoxic T cells (CTL) was determined in HNSCC patients. The frequency of CTL specific for the HLA-A2.1-restricted EGFR-derived YLN peptide (YLNTVQPTCV) and KLF peptide (KLFGTSGQKT) was determined in 16 HLA-A2.1+ HNSCC patients and 16 healthy HLA-A2.1+ individuals (NC) by multicolor flow cytometry. Patients' results were correlated to EGFR expression obtained by immunohistochemistry in corresponding tumor sections. Proliferation and anti-tumor activity of peptide-specific CTL was demonstrated by in vitro stimulation with dendritic cells pulsed with the peptides. Frequency of EGFR-specific CTL correlated significantly with EGFR expression in tumor sections (p = 0.02, r2 = 0.6). Patients with elevated EGFR scores (> 7) had a significantly higher frequency of EGFR-specific CTL than NC and patients with low EGFR scores (< 7). EGFR-specific CTL from cancer patients were expanded ex vivo and produced IFN-γ upon recognition of EGFR+ target cells. EGFR expressed on HNSCC cells induces a specific immune response in vivo. Strategies for expansion of EGFR-specific CTL may be important for future immunotherapy of HNSCC patients.

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Details

Item Type: Article
Status: Published
Creators/Authors:
CreatorsEmailPitt UsernameORCID
Schuler, PJ
Boeckers, P
Engers, R
Boelke, E
Bas, M
Greve, J
Dumitru, CA
Lehnerdt, GF
Ferris, RLrlf1@pitt.eduRLF1
Andrade Filho, PA
Brandau, S
Lang, S
Whiteside, TL
Hoffmann, TK
Centers: Other Centers, Institutes, Offices, or Units > Hillman Cancer Center
Date: 1 January 2011
Date Type: Publication
Journal or Publication Title: Journal of translational medicine
Volume: 9
Page Range: 168 - ?
DOI or Unique Handle: 10.1186/1479-5876-9-168
Refereed: Yes
Date Deposited: 26 Oct 2016 18:02
Last Modified: 04 Feb 2019 15:58
URI: http://d-scholarship.pitt.edu/id/eprint/30015

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