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Lineage relationship of prostate cancer cell types based on gene expression

Pascal, LE and Vêncio, RZ and Vessella, RL and Ware, CB and Vêncio, EF and Denyer, G and Liu, AY (2011) Lineage relationship of prostate cancer cell types based on gene expression. BMC Medical Genomics, 4.

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Background: Prostate tumor heterogeneity is a major factor in disease management. Heterogeneity could be due to multiple cancer cell types with distinct gene expression. Of clinical importance is the so-called cancer stem cell type. Cell type-specific transcriptomes are used to examine lineage relationship among cancer cell types and their expression similarity to normal cell types including stem/progenitor cells. Methods. Transcriptomes were determined by Affymetrix DNA array analysis for the following cell types. Putative prostate progenitor cell populations were characterized and isolated by expression of the membrane transporter ABCG2. Stem cells were represented by embryonic stem and embryonal carcinoma cells. The cancer cell types were Gleason pattern 3 (glandular histomorphology) and pattern 4 (aglandular) sorted from primary tumors, cultured prostate cancer cell lines originally established from metastatic lesions, xenografts LuCaP 35 (adenocarcinoma phenotype) and LuCaP 49 (neuroendocrine/small cell carcinoma) grown in mice. No detectable gene expression differences were detected among serial passages of the LuCaP xenografts. Results: Based on transcriptomes, the different cancer cell types could be clustered into a luminal-like grouping and a non-luminal-like (also not basal-like) grouping. The non-luminal-like types showed expression more similar to that of stem/progenitor cells than the luminal-like types. However, none showed expression of stem cell genes known to maintain stemness. Conclusions: Non-luminal-like types are all representatives of aggressive disease, and this could be attributed to the similarity in overall gene expression to stem and progenitor cell types. © 2011 Pascal et al; licensee BioMed Central Ltd.


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Item Type: Article
Status: Published
CreatorsEmailPitt UsernameORCID
Pascal, LElep44@pitt.eduLEP44
Vêncio, RZ
Vessella, RL
Ware, CB
Vêncio, EF
Denyer, G
Liu, AY
Date: 25 May 2011
Date Type: Publication
Journal or Publication Title: BMC Medical Genomics
Volume: 4
DOI or Unique Handle: 10.1186/1755-8794-4-46
Schools and Programs: School of Medicine > Urology
Refereed: Yes
Date Deposited: 28 Oct 2016 17:54
Last Modified: 04 Feb 2019 22:55


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